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Preparation method of polyvinyl alcohol immobilized cell spheroid carrier

A technology of immobilizing cells and polyvinyl alcohol, which is applied in the direction of being immobilized on/in an organic carrier to achieve the effects of low toxicity, good stability and simple operation

Inactive Publication Date: 2012-01-11
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to overcome the defects in the preparation of existing polyvinyl alcohol spherical carriers, the present invention provides a new preparation scheme, adding a small amount of sodium alginate to polyvinyl alcohol, and after dissolution, the cells do not contain or contain a small amount of Ca 2+ , SO 4 2- , NO 3 - The aqueous suspension is mixed with a certain concentration of Ca(NO 3 ) 2 Solution to replace saturated boric acid solution to coagulate polyvinyl alcohol composite gel, and freeze the formed particles in a low temperature environment. During the period, thaw and air-dry once to form fixed particles to solve the adhesion of polyvinyl alcohol particles in the polyvinyl alcohol boric acid method Expansion and H 3 BO 3 Toxicity to microorganisms while ensuring good performance of the granules

Method used

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Examples

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Embodiment 1

[0014] After culturing Thiobacillus ferrooxidans in 9K medium for 2-3 days, centrifuge the culture solution at 1500r / min for 5min, discard the precipitate, then centrifuge at 12000r / min for 15min, collect the cells, dissolve the cells with deionized water, and collect the cell suspension .

[0015] Weigh 3 groups of mixed gels according to the following proportions:

[0016] Polyvinyl alcohol (polymerization degree, 1750±50) 6g, sodium alginate (chemically pure) 1.0g;

[0017] Polyvinyl alcohol (polymerization degree, 1750±50) 8g, sodium alginate (chemically pure) 0.6g;

[0018] Polyvinyl alcohol (polymerization degree, 1750±50) 12g, sodium alginate (chemically pure) 0.2g;

[0019] Put the three groups of gels into 90ml of deionized water and heat to dissolve in a boiling water bath. After cooling to 40°C, add 10ml of Thiobacillus ferrooxidans cell suspension respectively, and the final cell concentration is 1×10 9 pc / ml, the concentrations of polyvinyl alcohol in the three...

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Abstract

The invention relates the fixation method of microbial cell. The method comprises the following steps: heating the polyvinyl alcohol and sodium alginate, dissolving them into water, cooling, mixing them with deionized water suspending liquid, dipping the mixture into 1%-5%(W / V) (W / V)Ca(NO3)2, dispensing for 0.5-2 hours, washing with deionized water, saving below -10Deg.C, and defreezing and seasoning at -3-3Deg.C, defreezing at room temperature, and getting the product. The method can be used for immobilization of microorganism and enzymes.

Description

Technical field [0001] The invention relates to a preparation technology of microbial cell immobilized particles, in particular to a new method for preparing polyvinyl alcohol spherical carriers. Background technique [0002] Polyvinyl alcohol, as a new type of microbial embedding and immobilization carrier, has a series of advantages such as high strength, good chemical stability, strong resistance to microbial decomposition, non-toxic to microorganisms, and low price. It is a kind of packaging with practical potential. Buried materials have been widely studied and applied at home and abroad in recent years. The currently widely used immobilization methods of polyvinyl alcohol are mainly boric acid method and freezing method. The general freezing method needs to be frozen and thawed many times, and finally cut into pieces to shape. method, but this method is prone to adhesion and swelling of gel balls, and boric acid is highly toxic to organisms, and cell activity will be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08
Inventor 李红玉王玉建支德娟
Owner LANZHOU UNIVERSITY
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