Extracorporeal cell culture model and process for HIV-1 infected CD4 cell

Abstract

The present invention is extracorporeal cell culture model and process for HIV-1 infected CD4+ cell, and relates to life science and medicine field. The present invention provides comprehensive HIV-1 infection treating mechanism for curing HIV-1 infection radically, designs new HIV-1 antagonizing medicine, provides new detection mechanism for detecting HIV-1 infection treating medicine, and provides relevant experiment reference. The present invention may be used in screening new HIV-1 antagonizing medicine for curing AIDS in early stage radically.

Description

technical field [0001] The present invention relates to an HIV-1 infected CD4 + In vitro cell culture models and methods. It involves the fields of life sciences and medicine. In order to be able to integrate scientific demonstration and simulation analysis to reveal: the comprehensive treatment mechanism that can cure early HIV-1 infection and effectively treat asymptomatic HIV-1 infection to prolong the survival period, design a new anti-HIV-1 that can theoretically cure early AIDS. 1 Drugs, formulating new detection mechanisms, methods, and standards for the detection and treatment of drugs for HIV-1 infected persons, human AIDS basic / clinical / medical comprehensive scientific research, providing relevant comprehensive scientific research and experimental basis. It can be applied to screening anti-HIV-1 drugs that can cure early AIDS in theory. Background technique [0002] Since the discovery of human immunodeficiency virus-AIDS in 1983, human beings have been in the f...

AI Technical Summary

Problems solved by technology

However, due to the generation of HIV drug-resistant strains, combined antiviral drug therapy eventually fails; because HIV-infected patients have 1% or more of the body, which is difficult or cannot be cleared by cellular immunity, long-term chronic HIV virus-infected cells , or static latent HIV virus infected cell reservoirs, which eventually lead to the failure of combined antiviral drug therapy; these are the two main reasons why the existing anti-HIV treatment cannot achieve the purpose of completely eliminating the virus in the patient's body and cannot cure HIV-infected patients

[0004] The inventor thinks: 1. Use reverse transcriptase inhibitors NRTIs / NNRTIs, or treat HIV-infected patients in combination with other anti-HIV-1 drugs, reverse transcriptase inhibitors interfere with viral RNA reverse transcription and replicate complementary cDNA strands, which will greatly increase the number of viruses The incidence of RNA reverse transcription replication DNA base mismatch, or loss, etc., produces more HIV-I gene mutation mutant strains, is the application of reverse transcriptase inhibitors NRTIs / NNRTIs to treat HIV-I infected patients, increase the production HIV-I mutant strains, drug-resistant mutant strains, important molecular biogenesis mechanisms that have not yet been revealed