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Chinese medicine active component composition and its preparing method and use

The technology of a composition and traditional Chinese medicine is applied in the application field of medicines or health care products for preventing and treating cardiovascular and cerebrovascular diseases, and can solve the problems of low phenolic acid content in extracts, unspecified phenolic acid content, large difference in proportions, and the like. The effect of high content of active ingredients, stable product quality and stable quality

Active Publication Date: 2007-04-11
兰州和盛堂药物研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Chinese patent, CN1247855A, disclosed in 2000 the method of extracting phenolic acids in salvia miltiorrhiza by resin method after water extraction, because the water extract has many impurities, resulting in low resin regeneration rate and low phenolic acid content in the extract
Chinese patent, CN1425659A, disclosed the method of extracting salvianolic acid B from salvianolic acid in 2003, which also has the above-mentioned defects, and salvianolic acid A, the most active active ingredient in salvianolic acid, has not been separated
Chinese patent, CN1513848A, disclosed in 2004 the method of extracting phenolic acids in salvia miltiorrhiza by means of water extraction, ultrafiltration, acidification, and extraction of acetates after acidification, and the extraction and extraction methods used in the literature It has been reported that the method of separation by reversed-phase column has been mentioned in the patent CN1425659A. This method does not explain how to specifically separate the components of phenolic acids in Salvia miltiorrhiza, and what is the content of the components of phenolic acids that are divided into also did not explain
[0010] At present, there are many reports on the pharmacological activity of the water-soluble components of Salvia miltiorrhiza, and there are many pharmacological studies on the main active components salvianolic acid A and salvianolic acid B, but there is no pharmacological study on the synergistic effect of the two, and the composition of traditional Chinese medicine is complex. , its efficacy is mostly the result of the synergistic effect of various chemical components. We have found through research that the contents of salvianolic acid A and salvianolic acid B in Danshen medicinal materials and different Danshen preparations and the ratio of the two are very different. The present invention finds that Salvia miltiorrhiza Phenolic acid A and salvianolic acid B have a synergistic effect on cardiovascular and cerebrovascular pharmacological effects, so the most reasonable composition of salvianolic acid A and salvianolic acid B based on pharmacodynamics is prepared

Method used

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  • Chinese medicine active component composition and its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1: the preparation method of salvianolic acid A and salvianolic acid B and composition thereof (respectively extracting and mixing method)

[0088] The specific process steps are as follows

[0089] 1. Extraction: Take Danshen decoction pieces, add 10 times the amount of 20% ethanol, soak at room temperature for 3 hours, then heat to 80-100°C for reflux extraction for 1.5 hours, separate the first extract, add 8 times the amount of 50% ethanol, and heat Reflux extraction at 80-100°C for 1 hour, and separate the second extract. The two extracts were combined, and the solid impurities in the extract were removed by filtration.

[0090] 2. Precipitation: Concentrate the extract under reduced pressure to a relative density of 1.05 to 1.10. After cooling, add deionized water 8 times the amount of medicinal materials, refrigerate, let stand, take the supernatant, and concentrate under reduced pressure to obtain a concentrate.

[0091] 3. Separation and purificati...

Embodiment 2

[0101] Embodiment 2: the preparation method of salvianolic acid A and salvianolic acid B and composition thereof, (simultaneous extraction method)

[0102] Other parts of the method are the same as in Example 1, and the column chromatography separation and purification process is different, and the column chromatography separation and purification method is as follows:

[0103] The pre-treated AB-8 macroporous adsorption resin column on the concentrated solution, the ratio of the sample on the column to the amount of resin is 1:8 (W:W), first eluted with deionized water for 20 column bed volumes to remove impurities, and then Elute 20 column bed volumes with 20% ethanol aqueous solution to remove impurities, then elute salvianolic acid B and salvianolic acid A with about 70% ethanol aqueous solution, and monitor salvianolic acid B in the eluent with TLC during the elution process and the content changes of salvianolic acid A, combined with HPLC monitoring, until the two are co...

Embodiment 3

[0104] Embodiment 3: the selection of pH in the preparation technology of sodium salt derivative

[0105] Take 100 g of the salvianolic acid A and B raw materials obtained in the above-mentioned Example 1, dissolve them in 500 ml of water, add 0.01 to 0.5 N sodium bicarbonate solution to adjust the pH to 6, 7, 8, 9, 10, 12, and 14 respectively, and fully After stirring, centrifuge to remove insoluble matter, concentrate the solution under reduced pressure, add ethanol to the alcohol concentration of 80%, let it stand still, filter the precipitate, and dry under reduced pressure to obtain the sodium salt of salvianolic acid A plus B, calculate the weight, and measure the salvia miltiorrhiza The contents of phenolic acid A sodium salt and salvianolic acid B sodium salt are shown in Table 1.

[0106] pH

[0107] The data in Table 1 shows that the influence of pH in the preparation process of salvianolic acid A+B sodium salt derivatives is very important: pH10, the conte...

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Abstract

The invention relates to a Chinese medicine composition of active components and its preparation method, the general amount of salvianolic A and salvianolic acid B, make up more than 50% thereof said composition , and the proportion between Sal A and Sal B is 1:0.01-100, said invention also provides application of said composition in preparation of medicine for treatment and prevention to and against blood vessel disease of heart and brain.

Description

technical field [0001] The invention relates to a composition composed of salvianolic acid A and salvianolic acid B, the active ingredients of the traditional Chinese medicine Salvia miltiorrhiza, and a preparation process thereof, a pharmaceutical preparation containing the composition, and a medicine for preventing and treating cardiovascular and cerebrovascular diseases or health care. product applications. Background technique [0002] Cardiovascular disease is a huge burden on global health care and health resources. According to the latest World Health Report released in 2000, 17 million people die from cardiovascular diseases in the world every year, and by 2020, cardiovascular deaths will increase by 50% to as high as 25 million. Cardiovascular and cerebrovascular diseases have become the number one killer of human health, with the highest incidence among middle-aged and elderly people. Its morbidity and mortality have surpassed tumor diseases and ranked first. It i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61K36/537A61P9/00B01D15/08A61K31/216
Inventor 王煜
Owner 兰州和盛堂药物研究院有限公司
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