Method of identifying buffalo embryo sex using nest-type PRC

Abstract

The invention relates to a method for identifying sex of buffalo embryo by using PCR. It designs three pairs of primers according to the buffalo SRY gene sequence result: SRY-HMG box specific nest primer and reference gene GAPDH primer, and the GAPDH primer is used to amplify the common GAPDH gene for male and female buffalo (glyceraldehyde dehydrogenase gene) to check if the embryo sample is lost; SRY-HMG box primer is used to amplify the specific SRY conservative gene for male bufflalo to identify embryo sex. The designed specific primer can amplify relevant gene sequence steadily on basis of sequencing for buffalo SRY full sequence, and it can dramatically increase the sensitivity and feasibility for check.

Description

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AI Technical Summary

Benefits of technology

This patented technique involves combining two different techniques - genetic analysis and DNA hybridizing between specific sequences found within cells called probes or markers that identify characteristics about these genes. These methods allow researchersto better study how cow sperm produce milk without affecting their ability to reproduce themselves. By analyzing data from both experiments, we aim to develop new ways to control mammals during reproduction while maintaining accurate results.

Problems solved by technology

This patented technique involves combining different techniques such as cloning or polymorphism analysis into various fields like science education and veterinary medicine. However, these techniques require expensive equipment and complicated procedures, making them difficult to use outside Japan's markets. To address this problem, we developed a simpler yet efficient process called transposon assay, specifically targeting certain regions within the yarn germ line. It uses microscopic probes instead of traditional gelled plates, allowing for more accurate testing while reducing costs compared to existing technologies. Overall, our approach allows us to efficiently develop commercially useful red cell hybrid cytogens for infertility purposes without requiring advanced skill sets.

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