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Process for preparing bioactive protein-enriched whey products

a technology of bioactive proteins and whey, which is applied in the field of process for preparing bioactive protein-enriched whey products, can solve the problems of high cost of isolation and purification of these bioactive proteins, and hinder their commercial utilization

Inactive Publication Date: 2005-09-22
PROLIANT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All of the above processes start with milk, whey, a combination or fraction thereof, and employ costly chromatographic or membrane processes to concentrate and / or purify bioactive proteins.
The high cost of isolation and purification of these bioactive proteins hinders their commercial utilization.

Method used

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  • Process for preparing bioactive protein-enriched whey products
  • Process for preparing bioactive protein-enriched whey products

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0045] Salt whey pressed out in Cheddar cheese process was collected from the tower. The salt whey was centrifuged at 7000×g for 15 minutes at 10° C. to remove fat. After adjusted to pH 6.2 with 6 N NaOH, the separated salt whey was concentrated by ultrafiltration and diafiltration using a bench UF unit with a 10K MWCO spiral-wound membrane cartridge. The final concentrate was freeze-dried into a protein powder (salt whey product A; Table 2).

example 2

[0046] The salt whey streams naturally drained from curd and pressed out in later cheese processing were pooled together and separated by a pilot plant separator (Westfalia SA-7). The separated salt whey was concentrated by ultrafiltration and diafiltration using a pilot plant UF unit with two 10K MWCO spiral-wound membranes. The final concentrate was spray-dried into a protein powder (salt whey product B; Table 2).

example 3

[0047] The salt whey pressed out in cheddar cheese process was collected from the tower. It was separated by a pilot plant separator (Westfalia SA-7). The separated salt whey was concentrated by ultrafiltration and diafiltration using a pilot plant UF unit with two 10K MWCO spiral-wound membranes. The final concentrate was spray-dried into a protein powder (salt whey product C; Table 2).

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Abstract

The present invention involves the discovery that various liquid (whey) streams drained or expelled from cheese curd after salt addition in the preparation of cheese contain enriched levels of bioactive proteins such as lactoferrin, lactoperoxidase, immunoglobulins, and growth factors. According to the invention, these proteins may be further enriched through manipulation of the cheese salting process as described herein. The methods of the invention may be used to produce various whey products with enriched levels of all the above bioactive proteins present and, through manipulation of salting conditions, to enrich these proteins selectively.

Description

BACKGROUND OF THE INVENTION [0001] The proteins present in milk, colostrum, whey, and other such compositions produced from lactating animals, are of great value. They have unique nutritional and functional properties and are often used as ingredients in processed and prepared foods, as well as nutritional supplements and even pharmaceutical formulations. These proteins are generally categorized into two classes. The first class is a heterogenous mixture called casein and represents approximately 80% of the proteins found in milk compositions. The second class is a heterogenous mixture called whey proteins comprising the remaining 20% of the proteins in milk. [0002] Human milk and bovine colostrum contain many bioactive whey proteins, such as lactoferrin (Lf), lactoperoxidase (Lp), immunoglobulins (Ig) and several growth factors. Bovine milk also contains these bioactive components, but at much lower levels than human milk and bovine colostrum. [0003] Large amount of research have b...

Claims

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Application Information

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IPC IPC(8): A23C21/00A23J1/20A23J3/08A23L1/305A61K8/64A61K8/98A61K35/20A61Q11/00A61Q19/00C07K14/495C07K14/65C12N5/00C12N9/08
CPCA23C21/00A23J1/205A23J3/08A23L1/3056C12N5/0018A61K8/986A61K35/20A61Q11/00A61Q19/00A61K8/64A23L33/19
Inventor WU, CHAOSTRUCKMEYER, TEDD
Owner PROLIANT
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