Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Muir-Torre-like syndrome in Fhit deficient mice

a mouse and fhit technology, applied in the field of tumor biology, can solve the problems that the acceptance of fhit as a tumor suppressor has not been universal, and achieve the effects of reducing increasing the tumor formation rate, and increasing the proliferation ra

Inactive Publication Date: 2006-04-06
CROCE CARLO +1
View PDF7 Cites 48 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nevertheless, acceptance of FHIT as a tumor suppressor has not been universal (Le Beau et al., 1998, Genes Chromosomes Cancer 21:281-289), with some reports suggesting that fragility of the locus alone could account for the occurrence of clonal or oligoclonal genetic alterations at FHIT in cancers.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Muir-Torre-like syndrome in Fhit deficient mice
  • Muir-Torre-like syndrome in Fhit deficient mice
  • Muir-Torre-like syndrome in Fhit deficient mice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The murine FHIT locus (FIG. 7; Pekarsky et al., 1998, Cancer Res. 58:3401-3408; Glover et al., 1998, Cancer Res. 58:3409-3414) is similar to its human homolog (U.S. Pat. No. 5,928,884), encompasses a common fragile site, and is altered in murine cancer cell lines. To define the role of FHIT protein in cancer development, a strain of Fhit + / − mice was established. The frequency of carcinogen-induced tumor formation in FHIT + / + and + / − mice was compared using the established N-nitrosomethylbenzylamine (NMBA) esophageal / gastric cancer model (Fong and Magee, 1999, Cancer Letters 143:63-69).

[0025] Upon bioactivation, NMBA produces benzaldehyde and an electrophilic methylating agent (Labuc and Archer, 1982, Cancer Res. 42:3181-3186), which methylates DNA, resulting in the formation of the promutagenic, adduct 06-methylguanine (O6-meG) (Fong et al., 1979, Int. J. Cancer 23:679-682). NMBA was reported to induce both esophageal and forestomach tumors when administered by gavage or in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides nonhuman transgenic animals with a disrupted FHIT gene. The invention further provides transgenic mice in which one or both Fhit alleles have been inactivated. Preferably, the Fhit-deficient mice develop multiple tumors of both visceral and sebaceous origin, similar to those of Muir-Torre familial cancer syndrome. The present invention further relates to the generation of these transgenic mice and their use as model systems to study the effects of carcinogenic agents in promoting clonal expansion of neoplastic cells in cancers, preferably gastrointestinal cancers of which Muir-Torre syndrome is a subset. The invention further relates to testing therapeutic agents for their efficacy in the prevention and treatment of cancer, preferably gastrointestinal cancer.

Description

[0001] This application claims the benefit of U.S. Provisional Application No. 60 / 196,534 filed Apr. 11, 2000, which is incorporated by reference herein in its entirety.[0002] This invention was made in part with government support under Grant numbers CA21124 and CA56336 awarded by the National Cancer Institute, USPHS; Grant number 97B115-REV from the American Institute for Cancer Research and Grant number ME99-105 from the Pennsylvania Department of Health. The Government has certain rights in the invention.1. FIELD OF THE INVENTION [0003] The present invention relates to the field of cancer biology, more particularly to transgenic animal that are predisposed to the development of multiple tumors and are useful as models for Muir-Torre familial cancer syndrome. 2. BACKGROUND OF THE INVENTION [0004] Since it was first noted that human chromosomal fragile sites mapped to chromosome bands that were nonrandomly altered by translocations or deletions in neoplasias, it has been proposed ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A01K67/027C12N5/06C07K14/47C07K14/82C12N5/02C12N5/10C12N15/09C12N15/85C12Q1/02G01N33/15G01N33/48G01N33/50
CPCA01K67/0276A01K2217/075A01K2217/20A01K2227/105A01K2267/0331C07K14/4703C07K14/82C12N15/8509C12N2503/02C12N2510/00C12N2517/02C12N2800/30
Inventor CROCE, CARLOHUEBNER, FRANCES
Owner CROCE CARLO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com