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Lactid acid bacteria as priobiotic strains and compositions containing same

a technology of lactic acid bacteria and composition, which is applied in the field of new probiotic bacteria, can solve the problems of common human urogenital and/or gastrointestinal infections, and the common problem of gastro-intestinal infections

Inactive Publication Date: 2010-08-05
FEDERICO GRAF
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

These probiotic strains effectively prevent and treat urogenital and gastrointestinal infections by establishing a healthy flora, inhibiting pathogen growth, and modulating the immune response, offering a natural and side-effect-free alternative to conventional treatments.

Problems solved by technology

Urogenital infections remain a common problem, particularly in the female population.
On the other hand gastro-intestinal infections remain a common problem in the human population.

Method used

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  • Lactid acid bacteria as priobiotic strains and compositions containing same

Examples

Experimental program
Comparison scheme
Effect test

example 1

1. Adhesion Capacity of L. Jensenii KS 119.1 and KS 130.1, L. Crispatus KS 116.1 and L. Gasseri KS 124.3 onto HeLa and Caco-2 / TC7 Cells

[0085]The level of adhesion of the above strains was determined after the cells were inoculated with four concentrations of lactobacilli (5×107; 1×108; 5×108; 1×109 CFU / well). Generally, a concentration-dependent adhesion was observed.

[0086]In cervix HeLa cells, adhesion levels observed show that all the tested strains are adhering. The L. jensenii KS 119.1 and KS 130.1 strains appeared the best adhering strains (7.5 log CFU / ml at 5×108 CFU / well) as compared with the control adhering strains, L. casei rhamnosus GG and L. rhamnosus GR1 strains.

[0087]In intestinal Caco-2 / TC7 cells, adhesion levels observed show that all the Medinova strains are adhering. The L. crispatus KS 116A, L. jensenii 119.1, 129.1 and KS 130.1, L. gasseri 124.3 strains appeared the best adhering strains (7.5-8 logs CFU / ml at 5×108 CFU / well) as compared with the control adhering ...

example 2

1. Activity of L. Gasseri KS 124.3, L. Helveticus KS 300 and L. Acidophilus KS 400 on the Growth of Urogenital and Intestinal Pathogens

[0106]It has been examined whether the strains referred to here above are active against the growth of Staphylococcus aureus, Streptococcus agalactiae, Candida albicans and uropathogenic and diarrheagenic E. coli strains IH11128 and 7372. The growth of pathogens was measured at 5, 8, 18 and 24 h.

[0107]No activity was developed against Streptococcus agalactiae and Candida albicans by L. gasseri KS 124.3, L. helveticus KS 300 and L. acidophilus KS 400 as well as by the control strains GR-I and RC-14. Concerning Staphylococcus aureus, the control L. rhamnosus strain GR-I and L. fermentum strain RC-14 efficiently inhibited the growth of the bacteria. Similarly, L. gasseri KS 124.3, L. helveticus KS 300 and L. acidophilus KS 400 inhibited the growth of Staphylococcus aureus and showed a decrease in the viable bacteria number. When activities of lactobacil...

example 3

1. Killing Activity of L. Jensenii KS 121.1 and KS 122.1, L. Gasseri KS 120.1 and L. Helveticus KS 300 Against Urogenital and Intestinal Pathogens

[0117]It has been examined whether said lactobacillus strains are active on the viability of Streptococcus agalactiae, Candida albicans, uropathogenic E. coli IH11128, Gardnerella vaginalis, Prevotella bivia and Salmonella enterica Typhimurium. The effect on viability of pathogens was measured at 4 h of contact.

[0118]Concerning both Streptococcus agalactiae and Candida albicans none of the tested lactobacilli showed an activity.

[0119]For uropathogenic E. coli strains IH11128, L. jensenii KS 121.1 and KS 122.1 showed no activity whereas, in contrast, L. gasseri KS 120.1 decreased efficiently (4 logs) the viability of E. coli in unshaken conditions. L. helveticus KS 300 and the L. fermentum RC-I control strain decreased of 2 logs the viability of E. coli in unshaken conditions only.

[0120]Concerning Gardnerella vaginalis, both L. jensenii KS ...

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Abstract

Useful probiotics have been selected among lactic acid bacteria strains of the genus L. acidophilus, L. crispatus, L. gasseri, L. helveticus and L. jensenii for their ability to kill urogenital and / or gastrointestinal pathogens and their ability to inhibit internalization of urogenital and / or gastrointestinal pathogens within urogenital and / or gastrointestinal epithelial cells in humans. Probiotic compositions comprise at least one of the said lactic acid bacteria strains in combination with a suitable delivery system, such as a food product or a beverage, a food or beverage compositions, a food or beverage supplement or adjuvant.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a U.S. National Phase of International Application No. PCT / EP2005 / 011152, filed Oct. 17, 2005, designating the U.S. and published in English on May 4, 2006 as WO 2006 / 045475, which claims the benefit of International Application No.: PCT / EP2004 / 0119810, filed Oct. 22, 2004; International Application No. PCT / EP2004 / 011981, filed Oct. 22, 2004; and International Application No. PCT / EP2005 / 001354, filed Feb. 10, 2005.FIELD OF THE INVENTION[0002]This invention refers to the use of new probiotic bacteria stains in the treatment of infectious troubles caused by various pathogens in mammals, more specifically the prevention and / or the treatment of urogenital and / or gastrointestinal infections in humans.BACKGROUND OF THE INVENTION[0003]Urogenital infections remain a common problem, particularly in the female population. Bacterial adherence to the urogenital epithelium is recognized as an important mechanism in the initiation a...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/74A61P1/00A61P13/02A61P31/00A61P15/02A61P13/10A61P29/00A61K35/747
CPCA23L1/3014A23L2/382A23V2002/00A61K9/02A61K35/747C12R1/225C12R1/23A61K2300/00A23V2200/32A23V2200/3204A23L33/135A61P1/00A61P13/00A61P13/02A61P13/10A61P15/00A61P15/02A61P29/00A61P31/00A61P31/04A61P31/10A61P37/02A61P37/04Y02A50/30C12R2001/23C12N1/205C12R2001/225A61K9/0034A61K9/0053A61K9/48A61K2035/115
Inventor GRAF, FEDERICOGROB, PHILIPPBRASSART, DOMINIQUE
Owner FEDERICO GRAF
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