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Methods for measuring the insulin receptor alpha subunit

Inactive Publication Date: 2011-01-20
MEDICAL & BIOLOGICAL LAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides methods for measuring the free insulin receptor α-subunit in blood, which can help evaluate the risk for diabetes. The invention also provides reagents and methods for diagnosing diabetes and cancer using the measurement of the free insulin receptor α-subunit.

Problems solved by technology

As a result, the insulin receptor α-subunit existing in the blood in a free form was revealed to hinder insulin action and cause hyperglycemia.

Method used

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  • Methods for measuring the insulin receptor alpha subunit
  • Methods for measuring the insulin receptor alpha subunit
  • Methods for measuring the insulin receptor alpha subunit

Examples

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Embodiment Construction

[0172]Hereinafter, the present invention will be described more specifically with reference to Examples.

1. Construction of Human Insulin Receptor α-Subunit cDNA

[0173]cDNA for causing secretion of insulin receptor α-subunit into the culture supernatant from CHO cells were constructed. The structures of the cDNAs constructed in the present example are shown in FIG. 1. Each of the cDNAs was obtained by using pcDL1-HIR717 (Ebina et al. Cell. 40, 747-758, 1985) comprising the full-length cDNA (NM—000208) of the human insulin receptor and digesting it at different positions. Each of the cDNAs has the following structures, respectively.

CHO-HIR: insulin receptor α-subunit+β-subunit

CHO-HIR(α): insulin receptor α-subunit alone

CHO-HIR(PstI): α-subunit and amino acids 1 to 150 in the N terminal side of the β-subunit

CHO-HIR(SspI): α-subunit and amino acids 1 to 191 in the N terminal side of the β-subunit

[0174]The β-subunit transmembrane domain is in positions 195 to 217 from the N-terminal side....

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Abstract

Presence of free insulin receptor α-subunit in blood was discovered. Furthermore, methods for measuring the insulin receptor α-subunit was provided, the method comprising the steps of contacting the insulin receptor α-subunit in a blood sample with an antibody recognizing the insulin receptor α-subunit, and detecting the binding between the two. Measurement of the free insulin receptor α-subunit in the blood is useful for evaluating risk factors for diabetes.In addition, the measurement methods of the present invention showed that concentrations of the free insulin receptor α-subunit in the blood of diabetes or cancer patients are significantly high. Free insulin receptor α-subunit in blood is useful as a marker for diabetes or cancer.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a divisional of U.S. application Ser. No. 10 / 554,561, filed Oct. 4, 2006, which is the U.S. National Stage entry under §371 of International Application No. PCT / JP2004 / 005412, filed Apr. 15, 2004, which claims benefit of Japanese Patent Application No. 2003-121955, filed Apr. 25, 2003 and Japanese Patent Application No. 2003-433303, filed Dec. 26, 2003; the disclosures of each are herein incorporated by reference in their entirety.TECHNICAL FIELD[0002]The present invention relates to methods for measuring a free insulin receptor α-subunit in blood. The present invention also relates to methods for diagnosing diabetes and cancer.BACKGROUND ART[0003]Insulin is a hormone that plays an important role in the metabolic regulation of glucose, an energy source for living organisms. Produced in pancreatic Langerhans β cells, insulin acts on cells carrying insulin receptors and promotes the uptake of glucose by these cells. The ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/53C07K16/28G01N33/68G01N33/74
CPCC07K16/2869G01N33/6893G01N2800/042G01N2333/62G01N2333/72G01N33/74
Inventor EBINA, YOUSUKEOBATA, TOSHIYUKIOKAMOTO, EIJI
Owner MEDICAL & BIOLOGICAL LAB CO LTD
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