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Inhibition of the Survival of Lymphoma by Cyclohexenone Compounds from Antrodia Camphorata

a technology of cyclohexenone and antrodia camphorata, which is applied in the direction of biocide, animal repellents, drug compositions, etc., can solve the problems of many adverse side effects or clinical discomfort symptoms, and the price of antrodia camphorata is very high, so as to inhibit the survival of lymphoma cells, prolong the treatment effect, and delay the growth of cancer cells

Inactive Publication Date: 2011-03-10
GOLDEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0015]Cyclohexenone compounds of the present invention are applied in inhibiting the survival of cancer cells, which can further be used as a pharmaceutical composition for treating cancer and to enhance the cancer therapeutic effects. The compounds of the invention can be applied in inhibiting the survival of lymphoma cells, which result in delaying the growth of the cancer cells and suppressing proliferation of the cancer cells, and further inhibiting cancer deterioration. The preferred compound is 4-hydroxy-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone of the formula (2).

Problems solved by technology

However, radiotherapy and chemotherapy usually lead to many adverse side effects or clinical uncomfortable symptoms.
The price of Antrodia camphorata is very high due to the extremely slow growth rate of natural Antrodia camphorata.

Method used

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  • Inhibition of the Survival of Lymphoma by Cyclohexenone Compounds from Antrodia Camphorata
  • Inhibition of the Survival of Lymphoma by Cyclohexenone Compounds from Antrodia Camphorata
  • Inhibition of the Survival of Lymphoma by Cyclohexenone Compounds from Antrodia Camphorata

Examples

Experimental program
Comparison scheme
Effect test

example 1

Isolation of 4-hydroxy-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone

[0020]One hundred grams of mycelia, fruiting bodies or mixture of both from Antrodia camphorata were placed into a flask. A proper amount of water and alcohol (70-100% ethanol solution) was added into the flask and were stirred at 20-25° C. for at least 1 hour. The solution was filtered through both a filter paper and a 0.45 μm membrane, and then collected as the extract.

[0021]The extract of Antrodia camphorata was subjected to High Performance Liquid chromatography (HPLC) analysis. The separation was performed on a RP18 column using a mobile phase consisted of methanol (A) and 0.1-0.5% acetic acid (B), with the gradient conditions: the ratio of (B) from 95% to 20% 0-10 minutes, from 20% to 10% 10-20 minutes, kept 10% 20-35 minutes, and increased from 10% to 95% 35-40 minutes at the flow rate of 1 ml / min. The column effluent was monitored with a UV-visible detector.

[0022]The frac...

example 2

In Vitro Survival Assay for Anti-Lymphoma Effects

[0023]Inhibiting effects of lymphoma cells by cyclohexenone compounds of Antrodia camphorata from example 1 were assessed according to the anticancer-drug screening model of National Cancer Institute (NCI). The compound 4-hydroxy-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone from example 1 was added into the culture media of lymphoma cell line U937 to determine the survival rates. Survival of cell was analyzed using MTT assay. U937 cell line was a human histiocytic lymphoma cell line.

[0024]MTT assay is commonly used to analyze cell proliferation, survival rate of viable cells and cytotoxicity. MTT (3[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) is a yellow dye which can be converted to water-insoluble purple formazan on the reductive cleavage of its tetrazolium ring by the succinate tetrazolium reductase in mitochondria of cells. The amount of formazan produced is used to detect the n...

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Abstract

The present invention relates to a novel application of a compound. The compound 4-hydroxy-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone of the invention is isolated and purified from the extracts of Antrodia camphorata, which can be applied for inhibiting the survival of lymphoma cells and be used as a pharmaceutical composition to inhibit the lymphoma tumor growth.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to a new application for inhibiting cancer cell survival, in particular to the application for inhibiting the survival of lymphoma cells by a compound isolated and purified from Antrodia camphorata. [0003]2. The Prior Arts[0004]Lymphoma is a malignant tumor, arising predominantly in lymphatic system comprising lymphocytes, lymphatic vessels and lymph nodes. The clinicopathological feature of lymphoma is that the damaged lymphocytes are replaced with immature lymphocytes. The pathological changes occurring in lymphatic organs including bone marrow, spleen and thymus, which are responsible for development, storage and differentiation of lymphocytes, can also induce lymphoma. According to different types of pathological tumor tissues, lymphoma can fall into two categories of Hodgkin's lymphoma and Non-Hodgkin's lymphoma, wherein Non-Hodgkin's lymphoma is the most common type of lymphoma.[0005]...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P35/00
CPCC07C49/753A61K31/122A61P35/00A61P35/02
Inventor LIU, SHENG-YUNWEN, WU-CHEKUO, MAO-TIEN
Owner GOLDEN BIOTECH
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