Reducing agent from microorganism belonging to genus bacillus and application for same
a technology of reducing agent and genus bacillus, which is applied in the field of reducing agent, can solve the problems of limited use of 70 ppm of reducing agent, inability to act on the generated metmyoglobin, and inability to meet the needs of medical ingredients containing bacteria, etc., and achieve the effect of improving the color tone of mea
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[0125]In order to find the substance which improves the color tone of meat, screening was carried out mainly on microorganism belonging to the genus Bacillus. The results of the experiments on the microorganism strains, which had been regarded as having high usefulness based on the result of screening, are described below.
[0126]1. Preparation of freeze-dried powder of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus natto, Bacillus thuringiensis, Bacillus mycoides
[0127]10 mL of the liquid medium shown in Table 1 was poured into a test tube, and sterilized at 120° C. for 20 minutes. As preculture, 1 Öse portion of Bacillus subtilis strain JCM1465 (=strain ATCC6051, IAM12118, and IFO13719), Bacillus amyloliquefaciens strain NBRC15535 (=strain ATCC23350), Bacillus natto, Bacillus thuringiensis strain NBRC13865 (=strain ATCC13366), and Bacillus mycoides strain IAM1190 (=strain IFO3039) each was inoculated in the test tube, and cultured overnight at 30° C. under shaking at 300 rp...
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