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Reducing agent from microorganism belonging to genus bacillus and application for same

a technology of reducing agent and genus bacillus, which is applied in the field of reducing agent, can solve the problems of limited use of 70 ppm of reducing agent, inability to act on the generated metmyoglobin, and inability to meet the needs of medical ingredients containing bacteria, etc., and achieve the effect of improving the color tone of mea

Inactive Publication Date: 2013-03-07
AMANO ENZYME INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent text describes a color tone improver that can be used to improve the color tone of meat or processed meat. The improver can enhance the color development, accelerate color development, or prevent color fading, resulting in a better quality meat with vivid colors.

Problems solved by technology

However, since nitrates and nitrites have acute toxicity to cause methemogulobinemia in human, their usage is limited to 70 ppm or less in terms of the residual nitrite.
However, the methods described in Patent Documents 1 and 2 cannot achieve sufficient color development effect, and the method described in Patent Document 3 uses nitrates contained in vegetables, and thus has a problem with safety.
These methods cannot act on metmyoglobin which has been generated, and cannot achieve sufficient color development effect or color tone holding effect.

Method used

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  • Reducing agent from microorganism belonging to genus bacillus and application for same
  • Reducing agent from microorganism belonging to genus bacillus and application for same
  • Reducing agent from microorganism belonging to genus bacillus and application for same

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[0125]In order to find the substance which improves the color tone of meat, screening was carried out mainly on microorganism belonging to the genus Bacillus. The results of the experiments on the microorganism strains, which had been regarded as having high usefulness based on the result of screening, are described below.

[0126]1. Preparation of freeze-dried powder of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus natto, Bacillus thuringiensis, Bacillus mycoides

[0127]10 mL of the liquid medium shown in Table 1 was poured into a test tube, and sterilized at 120° C. for 20 minutes. As preculture, 1 Öse portion of Bacillus subtilis strain JCM1465 (=strain ATCC6051, IAM12118, and IFO13719), Bacillus amyloliquefaciens strain NBRC15535 (=strain ATCC23350), Bacillus natto, Bacillus thuringiensis strain NBRC13865 (=strain ATCC13366), and Bacillus mycoides strain IAM1190 (=strain IFO3039) each was inoculated in the test tube, and cultured overnight at 30° C. under shaking at 300 rp...

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Abstract

The present invention is intended to provide a reducing agent effective for color development of meat and uses therefor. The present invention provides a reducing agent containing a heme reductase derived from a microorganism belonging to the genus Bacillus. Preferably, crushed bacterial cells of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus natto, Bacillus thuringiensis, or Bacillus mycoides are used.

Description

TECHNICAL FIELD[0001]The present invention relates to a reducing agent derived from a microorganism belonging to the genus Bacillus and uses thereof. The reducing agent of the present invention is particularly useful for improving the color tone of meat or processed meat. The present application claims priority based on Japanese Patent Application No. 2010-109779 filed on May 12, 2010, and the content of the patent application is hereby incorporated by reference herein in its entirety.BACKGROUND ART[0002]Meat color is an important factor for consumers to evaluate the meat quality. Bright red meat is regarded as good quality, and brown meat is regarded as stale. In this manner, the meat color markedly influences the consumers' willing to buy the meat and their evaluation of the meat.[0003]The color tone of meat reflects the proportion of myoglobin derivatives in the meat. Once myoglobin is oxidized to metmyoglobin, the color tone changes into brown, and thus the commercial value of t...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N9/02A23B4/22A61P7/00C12N9/06A61K38/44A23L5/41A23L13/00A23L13/40A61K35/74
CPCA23L1/3149A61K35/74A61K2035/11C12N9/0004A23L1/3002A23L1/272A23L1/30C12N9/0006C12N9/0051C12Y101/01284C12Y108/01004A23L1/27A23L5/46A23L13/00A23L5/40A23L5/41A23L33/10A23L33/105A23L13/48A61P43/00A61P7/00A61P9/08A61P9/10
Inventor OKUDA, KEITAYAMAGUCHI, SHOTARO
Owner AMANO ENZYME INC
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