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Method for in-vitro treatment of differentiated or undifferentiated cells by application electromagnetic fields

a technology of electromagnetic fields and in-vitro treatment, which is applied in the field of in-vitro cell treatment, can solve the problems of affecting both approaches, the complexity of the method, and the potential risks of the use of viral vectors

Inactive Publication Date: 2014-07-31
RINALDI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent describes a process for treating cells using low-power radiofrequency electromagnetic fields. The technical effect is that this process can help to differentiate or undifferentiated cells, leading to improved cell function and potentially better treatments for certain diseases.

Problems solved by technology

These approaches are therefore hampered both by the complexity of the method and, above all, by the potential risks associated with the use of viral vectors.
Moreover, with both reprogramming with intermediate iPS and direct reprogramming the differentiation yield obtained is extremely low, usually below 1%.
This low differentiation yield is furthermore associated with the high tumorigenic risk of the reprogrammed cell population remaining at a quasi-embryonic undifferentiated stage.

Method used

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  • Method for in-vitro treatment of differentiated or undifferentiated cells by application electromagnetic fields
  • Method for in-vitro treatment of differentiated or undifferentiated cells by application electromagnetic fields
  • Method for in-vitro treatment of differentiated or undifferentiated cells by application electromagnetic fields

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example 1

[0021]The device described in patent EP 1 301 241, which had been placed in a CO2 incubator, was regulated in such a way as to emit electromagnetic radiation at a frequency approximately equal to 2.4 GHz, and the convector electrodes were immersed in the culture medium wherein R1 mouse ES cells were already present.

[0022]The distance between the 2.4 Ghz frequency source and the culture medium was approximately 35 cm. The amount of electromagnetic radiation supplied was measured using a Tektronix 2754p spectrum analyser, by orientating the antenna thereof receiving most of the signal.

[0023]Taking into account a duration for each individual radiofrequency emission of 200 ms and a switch-off interval of 2.5 s, the following results were obtained: the emitted power P is approximately 2 mW, the electric field E is equal to 0.4 V / m, the magnetic field M is approximately 1 mA / m, and the specific absorption rate, or SAR, approximately 0.128 μW / g. Having ascertained α=1 A / Vm , and p=1000 Kg / ...

example 2

[0052]Multipotent human-adult mesenchymal stem cells (having a degree of differentiation potentiality less than that of the embryonic stem cells considered for definitions of pluri-or totipotentiality), isolated from adipose tissue and from other sources such as bone medulla, dental pulp and foetal membranes of full-term placenta, when exposed to the described magnetic field according to the invention for 72 h and then cultivated in the absence of further exposures for 4 or 7 days (corresponding to 7 or 10 days from starting time 0), behaved like quasi-embryonic, pluripotent cells in that they acquired the capacity to differentiate, on equal terms with mouse embryo cells exposed to the magnetic field of the present invention, both in myocardial cells, neuronal cells and skeletal-muscle cells. These results indicate that the treatment conducted as stated in the present invention is able to convert human stem elements from a state of multipotency to an extremely more “plastic” state o...

example 3

[0053]Human fibroblasts were exposed to a magnetic field for 72 h. and then cultured in the absence of exposure for a further 4 or 7 days (corresponding to 7 or 10 days from starting time 0) stock the duration of each individual radiofrequency emission was 200 ms with a switching-off interval of 2.5 s. Under these experimental conditions, the percentage of cells which expressed beta-3-tubulin, a marker of neuronal differentiation, was above 16%, while the percentage of cells expressing myoD, a marker of skeletal-muscle differentiation, was more than 20%, and the percentage of cells expressing alpha-sarcomeric actinin, a marker of terminal myocardial differentiation, was actually over 30%.

[0054]Application of the process according to the invention to human skin fibroblasts in culture was able to:[0055](i) induce at the transcriptional level, the activation of tissue-specific genes such as Mef2c, Tbx5, GATA4, Nkx2.5 and prodynorphin, associated with cardiogenic orientation,[0056](ii) ...

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Abstract

A process is described for the treatment of stem cells or differentiated cells by application of radiofrequency electro-magnetic fields, by means of an electromagnetic field generator (10), comprising a power supply (11), at least one antenna (12) adapted to radiate a scattered electromagnetic field (13) of a power less than 100 mW, a modulator (14) associated with said generator (10) and adapted to modulate the emission thereof, and at least one convector electrode (15), adapted to be direct the radiofrequency currents induced by said electromagnetic field (13), and applied in proximity of said stem or differentiated cells.

Description

FIELD OF THE INVENTION[0001]The present invention relates to the field of in-vitro cell treatment through the application of electromagnetic fields.PRIOR ART[0002]As is known, electromagnetic fields of various frequencies are widely used in the field of telecommunications, but in addition to this principal use, the aforementioned radiofrequencies have also recently proved capable of interfering with processes of cellular homoeostasis.[0003]In particular C. Ventura et al., in Turning on stem cell cardiogenesis with extremely low frequency magnetic fields (FASEB J. 19(1):155-157 (2005), have demonstrated that mouse embryonal stem (ES) cells exposed to extremely low frequency electromagnetic fields (50 Hz, 0.8 mTrms) considerably increased the transcription of cardiogenic and cardiospecific genes by raising the production of stem cell-derived cardiomyocytes.[0004]The reprogramming of both mouse and human adult somatic non-stem cells , such as fibroblasts, into induced pluripotent cells...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N13/00
CPCC12N5/0696C12N13/00C12N2529/00F04C2270/041
Inventor RINALDIFONTANI, VANIA
Owner RINALDI
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