Biodosimetry panels and methods

a biodosimetry and panel technology, applied in the field of assay methods, can solve the problems of increased mortality, dysfunction or fibrosis, and higher risk of cancer

Inactive Publication Date: 2014-10-23
THE HENRY M JACKSON FOUND FOR THE ADVANCEMENT OF MILITARY MEDICINE INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0042](b) comparing said level of said biomarkers in said sample to a level of said biomarkers in a normal control sample; and

Problems solved by technology

In addition, exposures to low or moderate doses (1-3 Gy) of ionizing radiation can result in increased mortality if accompanied by physical injuries, opportunistic infections, and / or hemorrhage.
Long-term effects include dysfunction or fibrosis in a wide range of organs and tissues, cataracts, and, ultimately, a higher risk of cancer.
Although radioactive material can be detected with instruments, assessment of the radiation dose or injury that a person has already received is more difficult.
Because current and foreseeable medical countermeasures for radiation injuries are often expensive, labor-intensive and time-consuming to administer (and monitor), have limited availability, and are occasionally associated with serious toxicities, they should only be administered to persons who will likely benefit from their use.
No rapid diagnostic exists that can reliably discriminate levels of IR exposure based on samples collected at a single time point.
The diagnostic “gold standard” in the field of radiation biodosimetry, the dicentric chromosome assay, is labor-intensive and slow, and its use in mass-casualty situations would be problematic.

Method used

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  • Biodosimetry panels and methods
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  • Biodosimetry panels and methods

Examples

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Methods

[0129]Assays.

[0130]Assays were developed as a number of different singleplex or multiplexed panels in MSD MULTI-ARRAY 96-well plates and analyzed using ECL detection on an MSD plate reader (such as the SECTOR or PR2 lines of plate readers available from Meso Scale Discovery, a division of Meso Scale Diagnostics, LLC, Gaithersburg, Md.). The biomarkers analyzed in the assay panels include the biomarkers listed in Table 1.

[0131]Prior to conducting an assay measurement with an assay panel, the sample was first diluted in an appropriate sample diluent to the specified dilution for that panel. The diluted sample (typically about 10 to 25 uL) was then combined with an additional volume of sample diluent (typically one to three times the diluted sample volume) in a well of a MULTI-ARRAY assay plate containing an array of capture antibodies for the targets in the panel. The plate was incubated for about 2 hours with shaking, sample was removed and the wells were washed three times wi...

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Abstract

The present invention relates to methods and kits to assess an absorbed dose of ionizing radiation and/or the severity of tissue injury from radiation in a patient. The invention also relates to algorithms used to calculate an absorbed dose of radiation based on biomarker measurements of a plurality of biomarkers that are altered relative to a normal control in the event of radiation exposure.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]The present application claims benefit of U.S. Provisional Application No. 61 / 540,584 filed on Sep. 29, 2011, the contents of all of which are incorporated herein by reference.STATEMENT REGARDING FEDERALLY-SPONSORED RESEARCH[0002]This invention was made with federal support under HHSO100201000009C awarded by the Department of Health and Human Services. The U.S. government has certain rights in the invention.FIELD OF THE INVENTION[0003]This application relates to assay methods, modules and kits for conducting diagnostic assays useful in the detection of radiation exposure and the severity of tissue injury to radiation.BACKGROUND OF THE INVENTION[0004]In the aftermath of an incident in which a significant number of civilians are exposed to radiation or radioactive materials, health authorities will need to be able to rapidly identify individuals who have been exposed to life-threatening significant doses of radiation. The deadly effects of ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/566
CPCG01N2800/40G01N33/566G01N33/6893G01N2800/60G01T1/02G01T1/00G16H20/40G01N27/3273G01N35/00G01N2333/70535G01N2333/70578
Inventor BLAKELY, WILLIAM FGLEZER, ELI N.KENTEN, JOHNKUMAR, SUDEEPMATHEW, ANUOSSETROVA, NATALIA I.SIGAL, GEORGE
Owner THE HENRY M JACKSON FOUND FOR THE ADVANCEMENT OF MILITARY MEDICINE INC
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