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Cosmetic composition for improving skin conditions comprising fusion protein

a technology of fusion protein and cosmetic composition, which is applied in the field of fusion protein, can solve the problems of limited application, side effects, irritation and redness, etc., and achieve the effects of excellent skin penetration, excellent skin penetration ability, and maintenance or enhancement of physiological synthesis

Active Publication Date: 2015-05-14
LG HOUSEHOLD & HEALTH CARE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is a new material that can penetrate skin and carry drugs through it. It also helps to make proteins that improve the health of the skin. This material is made by combining two other proteins, and this combination has the best ability to penetrate skin and support the growth of other important skin proteins.

Problems solved by technology

However, these active ingredients have a problem in that they may cause side effects, such as irritation and redness, upon application to the skin and thus have had limited applications.
Alternatively, these active ingredients have insignificant effects, and thus cannot provide a sufficient effect of improving skin conditions by promoting collagen synthesis in the skin.
It is known that this problem is mainly because the various active ingredients intended to exhibit wrinkle reduction effects by promoting collagen synthesis are not normally absorbed into the skin.
However, these peptides have a disadvantage in that they merely exhibit functionality by themselves and cannot be used as carriers for delivering other drugs.
This disadvantage suggests that excellent skin penetration alone does not satisfy the requirements for drug delivery.
However, the results of dedicated studies on such materials have not yet been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Selection of Skin-Penetrating Peptide

[0081]In order to select a skin-penetrating peptide, a phage display method consisting of a combination of elution test methods using a phage library and a transdermal agent was performed.

[0082]First, 109 phages derived from Ph.D-12 phage library kit (New England Biolab) were added to 500 μl of 1% BSA-containing TBS (50 mM Tris pH 7.5, 150 mM NaCl) solution to prepare a phage solution.

[0083]Then, the porcine skin (0.7 mm thickness, Medikinetics) was placed between the upper and lower ends of Franz glass cell (Standard diameter: 9 mm, Receiver: 5 ml, Permgear), and the phage solution was added thereto and allowed to react for 16 hours. Then, the phages that penetrate the porcine skin to reach a receiver at the lower end were collected and amplified.

[0084]The amplification was performed using E. coli ER2738 (New England Biolab) as a host cell. Specifically, 5 ml and of the phage solution was added to an E. coli ER2738 strain shake-cultured in 25 ml...

example 2

Preparation of Fusion Protein

Example 2-1

Preparation of Fusion Protein Comprising Skin-Penetrating Peptide and Human Growth Hormone (hGH)

[0087]The C-terminus of the skin-penetrating peptide having the amino acid sequence of SEQ ID NO: 1, obtained in Example 1, was linked to the N-terminus of a human growth hormone (hGH) having the amino acid sequence of SEQ ID NO: 2 by a linker consisting of two amino acids (GG), thereby preparing the fusion protein T-hGH (SEQ ID NO: 6).

[0088]Specifically, a polynucleotide encoding the amino acid sequence of SEQ ID NO: 1 and a polynucleotide encoding the amino acid sequence of SEQ ID NO: 2 were synthesized and linked to each other by a nucleotide sequence encoding two amino acids (GG), thereby constructing a polynucleotide sequence encoding the amino acid sequence of SEQ ID NO: 6. Meanwhile, a polynucleotide encoding the cleavage site DDDDK (Asp-Asp-Asp-Asp-Lys) that can be cleaved by enterokinase was synthesized and linked to the 5′-terminal end of ...

example 2-2

Preparation of Fusion Protein Comprising Skin-Penetrating Peptide and Epidermal Growth Factor (EGF)

[0090]In the same manner as described in Example 2-1 above, the C-terminus of the skin-penetrating peptide having the amino acid sequence of SEQ ID NO: 1, obtained in Example 1, was linked to the N-terminus of an epidermal growth factor (EGF) having the amino acid sequence of SEQ ID NO: 3 by a linker consisting of two amino acids (GG), thereby preparing the fusion protein T-EGF (SEQ ID NO: 7).

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Abstract

The present invention relates to a fusion protein comprising a skin-penetrating peptide, a polynucleotide encoding the fusion protein, an expression vector comprising the polynucleotide, a transformant comprising the expression vector, a method for preparing the fusion protein, a cosmetic composition for improving skin conditions, which comprises the fusion protein, and a pharmaceutical composition for external skin use, which comprises the fusion protein. The fusion protein of the invention comprises a skin-penetrating peptide bound to a physiologically active protein. The fusion protein significantly enhances the skin penetration and skin retention of the physiologically active protein while maintaining or enhancing the ability of the physiologically active protein to synthesize a material showing physiologically active effects. Thus, it can be widely used as an active ingredient in functional cosmetic compositions and pharmaceutical compositions for external skin use.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority to Korean Patent Application No. 10-2013-0138657, filed 14 Nov. 2013 (14 Nov. 2013); Korean Patent Application No. 10-2013-0138658, filed 14 Nov. 2013 (14 Nov. 2013); Korean Patent Application No. 10-2014-0020768, filed 21 Feb. 2014 (21 Dec. 2014); Korean Patent Application No. 10-2014-0020769, filed 21 Feb. 2014 (21 Feb. 2014); Korean Patent Application No. 10-2014-0020770, filed 21 Feb. 2014 (21 Feb. 2014); and Korean Patent Application No. 10-2014-0020771, filed 21 Feb. 2014 (21 Feb. 2014), the disclosure of which are incorporated herein by reference in their entirety.FIELD OF THE INVENTION[0002]The present invention relates to a fusion protein comprising a skin-penetrating peptide, a polynucleotide encoding the fusion protein, an expression vector comprising the polynucleotide, a transformant comprising the expression vector, a method for preparing the fusion protein, a cosmetic composition for improvi...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07K14/50A61Q19/08A61K8/64C07K14/485C07K14/49
CPCC07K14/50C07K14/485C07K14/49A61K2800/10A61Q19/08A61K38/00A61K8/64C07K7/08A61Q19/007A61Q19/02C07K14/503C07K14/61C07K14/615C07K2319/00C07K2319/01
Inventor LEE, SEOL HOONLEE, SANG HWAKANG, NAE GYUHUR, EU GENE
Owner LG HOUSEHOLD & HEALTH CARE LTD
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