Cosmetic composition for improving skin conditions comprising fusion protein

a technology of fusion protein and cosmetic composition, which is applied in the field of fusion protein, can solve the problems of limited application, side effects, irritation and redness, etc., and achieve the effects of excellent skin penetration, excellent skin penetration ability, and maintenance or enhancement of physiological synthesis

Active Publication Date: 2015-05-14
LG HOUSEHOLD & HEALTH CARE LTD
View PDF0 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0008]Accordingly, the present inventors have made extensive efforts to develop novel materials satisfying two requirements: excellent skin penetration, and the maintenance or enhancement of synthesis of physiologically active materials in the skin. As a result, the present inventors have developed a skin-penetrating peptide, which can be used as a carrier for transdermal delivery of drugs and, at the same time, can remain in the skin, and have found that a fusion protein comprising the skin-penetrating peptide and a physiologically active protein has excellent skin penetration ability while maintaining or enhancing the synthesis of physiologically active materials, such as collagen, elastin, laminin and HAS2, in the skin, thereby completing the present invention.

Problems solved by technology

However, these active ingredients have a problem in that they may cause side effects, such as irritation and redness, upon application to the skin and thus have had limited applications.
Alternatively, these active ingredients have insignificant effects, and thus cannot provide a sufficient effect of improving skin conditions by promoting collagen synthesis in the skin.
It is known that this problem is mainly because the various active ingredients intended to exhibit wrinkle reduction effects by promoting collagen synthesis are not normally absorbed into the skin.
However, these peptides have a disadvantage in that they merely exhibit functionality by themselves and cannot be used as carriers for delivering other drugs.
This disadvantage suggests that excellent skin penetration alone does not satisfy the requirements for drug delivery.
However, the results of dedicated studies on such materials have not yet been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

Selection of Skin-Penetrating Peptide

[0081]In order to select a skin-penetrating peptide, a phage display method consisting of a combination of elution test methods using a phage library and a transdermal agent was performed.

[0082]First, 109 phages derived from Ph.D-12 phage library kit (New England Biolab) were added to 500 μl of 1% BSA-containing TBS (50 mM Tris pH 7.5, 150 mM NaCl) solution to prepare a phage solution.

[0083]Then, the porcine skin (0.7 mm thickness, Medikinetics) was placed between the upper and lower ends of Franz glass cell (Standard diameter: 9 mm, Receiver: 5 ml, Permgear), and the phage solution was added thereto and allowed to react for 16 hours. Then, the phages that penetrate the porcine skin to reach a receiver at the lower end were collected and amplified.

[0084]The amplification was performed using E. coli ER2738 (New England Biolab) as a host cell. Specifically, 5 ml and of the phage solution was added to an E. coli ER2738 strain shake-cultured in 25 ml...

example 2

Preparation of Fusion Protein

Example 2-1

Preparation of Fusion Protein Comprising Skin-Penetrating Peptide and Human Growth Hormone (hGH)

[0087]The C-terminus of the skin-penetrating peptide having the amino acid sequence of SEQ ID NO: 1, obtained in Example 1, was linked to the N-terminus of a human growth hormone (hGH) having the amino acid sequence of SEQ ID NO: 2 by a linker consisting of two amino acids (GG), thereby preparing the fusion protein T-hGH (SEQ ID NO: 6).

[0088]Specifically, a polynucleotide encoding the amino acid sequence of SEQ ID NO: 1 and a polynucleotide encoding the amino acid sequence of SEQ ID NO: 2 were synthesized and linked to each other by a nucleotide sequence encoding two amino acids (GG), thereby constructing a polynucleotide sequence encoding the amino acid sequence of SEQ ID NO: 6. Meanwhile, a polynucleotide encoding the cleavage site DDDDK (Asp-Asp-Asp-Asp-Lys) that can be cleaved by enterokinase was synthesized and linked to the 5′-terminal end of ...

example 2-2

Preparation of Fusion Protein Comprising Skin-Penetrating Peptide and Epidermal Growth Factor (EGF)

[0090]In the same manner as described in Example 2-1 above, the C-terminus of the skin-penetrating peptide having the amino acid sequence of SEQ ID NO: 1, obtained in Example 1, was linked to the N-terminus of an epidermal growth factor (EGF) having the amino acid sequence of SEQ ID NO: 3 by a linker consisting of two amino acids (GG), thereby preparing the fusion protein T-EGF (SEQ ID NO: 7).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
temperatureaaaaaaaaaa
temperatureaaaaaaaaaa
pHaaaaaaaaaa
Login to view more

Abstract

The present invention relates to a fusion protein comprising a skin-penetrating peptide, a polynucleotide encoding the fusion protein, an expression vector comprising the polynucleotide, a transformant comprising the expression vector, a method for preparing the fusion protein, a cosmetic composition for improving skin conditions, which comprises the fusion protein, and a pharmaceutical composition for external skin use, which comprises the fusion protein. The fusion protein of the invention comprises a skin-penetrating peptide bound to a physiologically active protein. The fusion protein significantly enhances the skin penetration and skin retention of the physiologically active protein while maintaining or enhancing the ability of the physiologically active protein to synthesize a material showing physiologically active effects. Thus, it can be widely used as an active ingredient in functional cosmetic compositions and pharmaceutical compositions for external skin use.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority to Korean Patent Application No. 10-2013-0138657, filed 14 Nov. 2013 (14 Nov. 2013); Korean Patent Application No. 10-2013-0138658, filed 14 Nov. 2013 (14 Nov. 2013); Korean Patent Application No. 10-2014-0020768, filed 21 Feb. 2014 (21 Dec. 2014); Korean Patent Application No. 10-2014-0020769, filed 21 Feb. 2014 (21 Feb. 2014); Korean Patent Application No. 10-2014-0020770, filed 21 Feb. 2014 (21 Feb. 2014); and Korean Patent Application No. 10-2014-0020771, filed 21 Feb. 2014 (21 Feb. 2014), the disclosure of which are incorporated herein by reference in their entirety.FIELD OF THE INVENTION[0002]The present invention relates to a fusion protein comprising a skin-penetrating peptide, a polynucleotide encoding the fusion protein, an expression vector comprising the polynucleotide, a transformant comprising the expression vector, a method for preparing the fusion protein, a cosmetic composition for improvi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(United States)
IPC IPC(8): C07K14/50A61Q19/08A61K8/64C07K14/485C07K14/49
CPCC07K14/50C07K14/485C07K14/49A61K2800/10A61Q19/08A61K38/00A61K8/64C07K7/08A61Q19/007A61Q19/02C07K14/503C07K14/61C07K14/615C07K2319/00C07K2319/01
Inventor LEE, SEOL HOONLEE, SANG HWAKANG, NAE GYUHUR, EU GENE
Owner LG HOUSEHOLD & HEALTH CARE LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap