The invention discloses a children hand sanitizer and a preparation method thereof. The children hand sanitizer is prepared from the following raw materials: glycerin, hyaluronic acid, mint oil, citric acid, vegetable oil, garlic essential oil, essence, traditional Chinese medicine liquid, a chelating agent, a surfactant, pearl powder, table salt and pure water. The children hand sanitizer can effectively remove dirt on a hand surface, cleans hand skin, improves hand skin, enables the hand skin to be smooth, white and tender, further has the effects of sterilizing and disinfecting, and guarantees the hand to be clean and sanitary. Besides, the components of the hand sanitizer provided by the invention are reasonably matched, act mutually, can effectively kill various bacteria, are long insterilizing and disinfecting effect, are safe and efficient, protect the hand skin, and do not cause harm on the environment and a human body.