The application discloses a display panel and a compensation data determination method, a determination device and a driving method thereof. The compensation data determination method comprises the following steps: providing driving data to the display panel for at least twice, making the display panel display corresponding display pictures for at least twice, and acquiring optical data corresponding to the display pictures and compensation data corresponding to the optical data, respectively; wherein, in the driving data provided for at least twice, the corresponding driving data in the latter time is obtained according to the driving data in the former time and the compensation data corresponding to the optical data in the former time; and finally, the final compensation data corresponding to the display panel is determined according to the compensation data corresponding to the optical data for at least twice. The application provides a display panel and a compensation data determination method, a determination device and a driving method thereof, which can effectively improve the display unevenness of the display panel and improve the display effect of the display panel.