The invention discloses a skin care composition with moisturizing and wrinkle-resistant effects. The composition is prepared by using sterile deionized water as a solvent, and the solute comprises amino acids, vitamins and trace elements including glycine, phenylalanine, alanine, proline, glutamine, serine, arginine, threonine, asparagine, tryptophan, aspartic acid, tyrosine, cysteine, valine, cystine, vitamin H, glutamic acid, nicotinamide, histidine, vitamin B6, isoleucine, vitamin B2, leucine, and vitamin B1. The skin care composition has good moisturizing and wrinkle-resistant effects, and has the advantages of safety without side effect, skin nourishing and good skin penetration capacity.