Gel gent of Chiense forest frog bactericidal peptide and its preparation process

Abstract

A tree frog's antibacterial peptide gel for treating the dermatopathy caused by aeruginose pseudomonads and the infection of burn and scald is prepared from antibacterial peptide for tree frog, carboxymethyl cellulose sodium glycerine, sodium dodecyl sulfonate, ethylester of nipagin and water through preparing transparent gel matrix and adding antibacterial peptide of tree frog.

Description

technical field

[0001] The invention belongs to the field of an antibacterial and analgesic drug and its preparation, in particular to a Rana antibacterial peptide drug and a method for preparing a Rana antibacterial peptide gel. Background technique

[0002] Antimicrobial peptides (AMPs) are an important part of the internal defense system of organisms. Antimicrobial peptides have the ability to resist Gram-positive bacteria, Gram-negative bacteria, fungi, coated viruses, protists and drug-resistant bacteria, and have been widely used in food preservation and medicine fields.

[0003] Amphibians live in complex and diverse environments, and their skin plays an important role in maintaining their survival, colonizing and adapting to their vast habitats. In order to adapt to the living environment, a wide variety of active substances with complex functions are distributed in the skin of amphibians, which is a huge resource bank of active biomolecules and drug resources. In 1...

Examples

Embodiment 1

[0025] Example 1 Effect of Rana antimicrobial peptide dosage on antibacterial activity

[0026] Select 10 grams of sodium carboxymethylcellulose, 64 grams of glycerin, 0.8 grams of sodium dodecyl sulfonate (SDS), and 2.0 grams of ethylparaben. Add appropriate amount of water for injection to sodium carboxymethyl cellulose to swell, add glycerin to grind and moisten, then add SDS to grind to obtain a transparent gel matrix; add ethyl paraben to appropriate amount of water for injection, and heat to 40-50°C Make it all dissolve, add it to the gel matrix, add water to a sufficient amount, stir evenly, and sterilize at high temperature. The prepared gel matrix is ​​divided into four parts for use; 10 grams, 15 grams, 30 grams , 40 grams are respectively added in four parts of the gel matrix, stirred evenly, and mixed with the Rana antimicrobial peptide gels which account for about 10%, 15%, 30%, and 40% of the Rana antimicrobial peptide by mass.

[0027] The antibacterial activit...

Embodiment 2

[0036] 1. The optimal dosage of raw materials and excipients of Rana antimicrobial peptide gel is as follows:

[0037] (1) sodium carboxymethylcellulose is 3%;

[0038] (2) Glycerin is 15%;

[0039] (3) SDS is 0.25%;

[0040] (4) Ethylparaben is 0.2%;

[0041] (5) Antimicrobial peptides are 22%;

[0042] (6) The balance is water for injection.

[0043] 2. Zone of inhibition test

[0044] (1) Strains

[0045] Pseudomonas aeruginosa ATCC 27853

[0046] Escherichia coli ATCC 25922

[0047] Staphylococcus aureus Staphylocouus aureus ATCC 25923

[0048] Pseudomonas aeruginosa with clinical multi-drug resistance (the main drug-resistant names are piperacillin, ofloxacin, cefotaxime, sulfamethoxazole, third-generation cephalosporin, and peridone)

[0049] Clinical multidrug-resistant Staphylococcus aureus (main drug-resistant names are penicillin, erythromycin, ofloxacin, methicillin)

[0050] (2) Method

[0051] Cultivate the above-mentioned strains to the logarithmic gro...

technical field

[0001] The invention belongs to the field of an antibacterial and analgesic drug and its preparation, in particular to a Rana antibacterial peptide drug and a method for preparing a Rana antibacterial peptide gel. Background technique

[0002] Antimicrobial peptides (AMPs) are an important part of the internal defense system of organisms. Antimicrobial peptides have the ability to resist Gram-positive bacteria, Gram-negative bacteria, fungi, coated viruses, protists and drug-resistant bacteria, and have been widely used in food preservation and medicine fields.

[0003] Amphibians live in complex and diverse environments, and their skin plays an important role in maintaining their survival, colonizing and adapting to their vast habitats. In order to adapt to the living environment, a wide variety of active substances with complex functions are distributed in the skin of amphibians, which is a huge resource bank of active biomolecules and drug resources. In 1...

Examples

Embodiment 1

[0025] Example 1 Effect of Rana antimicrobial peptide dosage on antibacterial activity

[0026] Select 10 grams of sodium carboxymethylcellulose, 64 grams of glycerin, 0.8 grams of sodium dodecyl sulfonate (SDS), and 2.0 grams of ethylparaben. Add appropriate amount of water for injection to sodium carboxymethyl cellulose to swell, add glycerin to grind and moisten, then add SDS to grind to obtain a transparent gel matrix; add ethyl paraben to appropriate amount of water for injection, and heat to 40-50°C Make it all dissolve, add it to the gel matrix, add water to a sufficient amount, stir evenly, and sterilize at high temperature. The prepared gel matrix is ​​divided into four parts for use; 10 grams, 15 grams, 30 grams , 40 grams are respectively added in four parts of the gel matrix, stirred evenly, and mixed with the Rana antimicrobial peptide gels which account for about 10%, 15%, 30%, and 40% of the Rana antimicrobial peptide by mass.

[0027] The antibacterial activit...

Embodiment 2

[0036] 1. The optimal dosage of raw materials and excipients of Rana antimicrobial peptide gel is as follows:

[0037] (1) sodium carboxymethylcellulose is 3%;

[0038] (2) Glycerin is 15%;

[0039] (3) SDS is 0.25%;

[0040] (4) Ethylparaben is 0.2%;

[0041] (5) Antimicrobial peptides are 22%;

[0042] (6) The balance is water for injection.

[0043] 2. Zone of inhibition test

[0044] (1) Strains

[0045] Pseudomonas aeruginosa ATCC 27853

[0046] Escherichia coli ATCC 25922

[0047] Staphylococcus aureus Staphylocouus aureus ATCC 25923

[0048] Pseudomonas aeruginosa with clinical multi-drug resistance (the main drug-resistant names are piperacillin, ofloxacin, cefotaxime, sulfamethoxazole, third-generation cephalosporin, and peridone)

[0049] Clinical multidrug-resistant Staphylococcus aureus (main drug-resistant names are penicillin, erythromycin, ofloxacin, methicillin)

[0050] (2) Method

[0051] Cultivate the above-mentioned strains to the logarithmic gro...