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Method for breeding halophyte of double transgene

A gene and plant technology, applied in the field of cultivation of double transgenic salt-tolerant and drought-resistant plants, can solve problems such as the inability to desalinize the land and the lack of agricultural application research value.

Inactive Publication Date: 2007-12-19
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI +1
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Problems solved by technology

[0004] The first method mentioned above has little value in agricultural application research, because plants do not absorb the salt in the soil, and a large amount of salt remains in the soil, so that the land cannot be desalinated; the second mechanism involves the shape of the plant, The structure and physiological metabolism are very complex. Plants mainly adopt direct or indirect protective mechanisms to improve their salt resistance, that is, to use the metabolism of small molecule osmotic adjustment substances and overexpression of genes that can scavenge active oxygen free radicals to improve plant immunity. tolerance to salt stress

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  • Method for breeding halophyte of double transgene
  • Method for breeding halophyte of double transgene
  • Method for breeding halophyte of double transgene

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[0021] specific implementation plan

[0022] One aspect of the present invention relates to a method of cultivating a plant variety with improved stress resistance, the method comprising:

[0023] a) Genes related to the synthesis of small molecule osmoregulatory substances or genes that scavenge active oxygen free radicals or their biologically active fragments or analogs, and Na + / H + Transforming the exchange protein gene or its biologically active fragment or analogue into the plant cell, and expressing the gene in the plant cell;

[0024] b) regenerating plants from the plant cells obtained in step a), and selecting plant varieties with improved stress resistance.

[0025] In the terms used in the present invention, "target gene" refers to the gene related to the synthesis (accumulation) of small molecule osmotic adjustment substances and the gene that can scavenge active oxygen free radicals (hereinafter referred to as "target gene A"), Na + / H + Exchange protein (N...

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Abstract

A method for culturing the salt-resistant dual-transgenic plant with high stress resistant includes such steps as transferring such genes in the plant cell as the gene associated with synthesizing macro-molecular osmosis regulator, the gene for removing the free radicals of active oxygen or its bioactive fragment, and Na / H exchanging protein gene or its bioactive fragment, expressing said gene in the plant, and regenerating the plant from the obtained plant cell.

Description

technical field [0001] The invention relates to a method for cultivating double-transgenic salt-tolerant and drought-resistant plants, more specifically a method for cultivating double-transgenic tobacco, tomato, rapeseed and other salt-resistant and drought-resistant plants. Background technique [0002] The salinization of the land has seriously restricted the development of modern agriculture. In order to breed new crop varieties with salt-tolerant characteristics, scientists around the world have conducted a lot of research. The results show that the damage caused by salt stress to plants is mainly due to the change of plant cell osmotic pressure causing cell water loss and excessive salt blocking the main chemical metabolic pathways in plant cells. There is a large amount of salt in the cells of natural halophyte, but various enzymes extracted from these plant cells are very sensitive to salt ions (Flowers 1977; Glenn et al.1999), indicating that these plants can The a...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82A01H4/00C12N15/87C12N5/10
Inventor 张洪霞杨磊贾同春
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI