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Gene of chlorine tolerance strain V430

A strain and gene technology, applied in genetic engineering, plant genetic improvement, bacteria, etc., can solve the problems of time-consuming and laborious identification of physiological and chemical characteristics, and difficulty in determining attribution.

Inactive Publication Date: 2008-09-17
CHINA UNIV OF GEOSCIENCES (WUHAN)
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Problems solved by technology

[0005] Due to the chlorine-resistant characteristics of chlorine-resistant bacteria, their morphology, cell wall, intracellular protein, fatty acid and other macromolecular compositions will change to varying degrees in the presence of salt. It is time-consuming and labor-intensive to judge their belonging, and it is very time-consuming and labor-intensive to identify them from the physiological and chemical characteristics, especially for a large number of isolated strains

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  • Gene of chlorine tolerance strain V430
  • Gene of chlorine tolerance strain V430
  • Gene of chlorine tolerance strain V430

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Embodiment Construction

[0060] The present invention will be further described below in conjunction with the embodiments and accompanying drawings.

[0061] The gene of Staphylococcus saprophyticus V430CCTCC NO.M205145 provided by the present invention, its 16S rDNA complete sequence is as follows figure 1 shown.

[0062] Using the BLAST analysis method, the 16S rDNA complete sequence of the strain Staphylococcus saprophyticus V430CCTCC NO.M205145 was compared with the GenBank database, and the homology between the strain and Staphylococcus saprophyticus was 98.0-99.0%, so it can be determined from the molecular level The strain was Staphylococcus saprophyticus. At the same time, combined with the morphological and physiological and biochemical characteristics obtained by the classical classification method, it was finally determined that the strain was Staphylococcus saprophyticus.

[0063] The present invention adopts extraction of bacterial nuclear DNA, PCR amplification of 16S rDNA gene, recove...

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Abstract

The chlorine tolerant strain gene V430 is the 16S rDNA gene of Staphylococcus saprophyticus V430. The gene of the present invention is obtained through extracting the nucleoplasm DNA, PCR amplification of 16S rDNA gene, recovery of PCR product, and whole 16S rDNA sequence determination and analysis. The present invention has the advantages of high speed and being simple.

Description

technical field [0001] The present invention relates to the gene of chlorine-resistant bacterial strain V430, which is the 16S rDNA gene of saprophytic staphylococcus (Staphylococcus saprophyticus) V430. Among them, Staphylococcus saprophyticus V430 has been preserved by China Center for Type Culture Collection (CCTCC for short), the preservation date is December 9, 2005, and the preservation number is CCTCC NO.M205145. Through the combination of classical phenotype identification and 16S rDNA nucleotide sequence analysis, the chlorine-resistant strain V430 was identified at the physiological, biochemical and 16S rDNA molecular levels, thus confirming that it was Staphylococcus saprophyticus. Background technique [0002] In nature, there are some microorganisms that can grow normally in environments containing high or even extremely high concentrations of chloride ions. After research, it was found that not all of these microorganisms can be applied to the treatment of was...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/31C12N1/20
Inventor 信欣王焰新鲍建国刘慧李平杨雪芬
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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