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Method of extracting soluble polysaccharide in bitter vegetable

A technology of soluble and bitter herbs, which is applied in the field of extracting soluble polysaccharides from bitter herbs of Patrinaceae, achieving the effects of simple process, high extraction rate and high purity

Inactive Publication Date: 2008-10-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports at home and abroad on the extraction process of bitter vegetable polysaccharides.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1) Drying and crushing: 100 g of fresh bitter herbs are cleaned and dried, dried in an oven, and finally crushed with a plant grinder to obtain 12.1 g of bitter herbs powder;

[0013] 2) Lixiviation: After mixing the above-mentioned bitter vegetable powder and distilled water in a weight ratio of 1:15, hot-leaching in a hot water bath at 80° C. for 4 hours, cooling and filtering;

[0014] 3) centrifugation: centrifuging the above-mentioned filtrate to obtain a liquid phase and a precipitate;

[0015] 4) Washing: Mix the liquid phase with 80% alcohol at a weight ratio of 1:5, stir to obtain a washing liquid, and repeat this twice;

[0016] 5) Drying: vacuum-dry the above-mentioned washing solution to obtain 0.0786 g of bitter vegetable polysaccharide, wherein the polysaccharide has a purity of 55%.

Embodiment 2

[0018] 1) Drying and crushing: 100 g of fresh bitter herbs are cleaned and dried, dried in an oven, and finally crushed with a plant grinder to obtain 12.0 g of bitter herbs powder;

[0019] 2) Lixiviation: After mixing the above-mentioned bitter vegetable powder and distilled water in a weight ratio of 1:20, heat leaching in a hot water bath at 100° C. for 6 hours, and filter after cooling;

[0020] 3) centrifugation: centrifuge the above-mentioned filtrate to a constant volume to obtain a liquid phase and a precipitate;

[0021] 4) Washing: Mix the liquid phase I with 80% alcohol in a weight ratio of 1:10, stir to obtain a washing liquid, and repeat this 4 times;

[0022] 5) Drying: vacuum-dry the above-mentioned washing solution to obtain 0.310 g of bitter vegetable polysaccharide, wherein the polysaccharide has a purity of 70%.

Embodiment 3

[0024] 1) Drying and crushing: 100 g of fresh bitter herbs are cleaned and dried, dried in an oven, and finally crushed with a plant grinder to obtain 12.1 g of bitter herbs powder;

[0025] 2) Lixiviation: After mixing the above-mentioned bitter vegetable powder and distilled water in a weight ratio of 1:40, heat leaching in a hot water bath at 90° C. for 6 hours, and filter after cooling;

[0026] 3) centrifugation: centrifuge the above-mentioned filtrate to a constant volume to obtain a liquid phase and a precipitate;

[0027] 4) Washing: Mix the liquid phase I with 80% alcohol in a weight ratio of 1:8, stir to obtain a washing liquid, and repeat this 3 times;

[0028] 5) Drying: vacuum-dry the above washing solution to obtain 0.265 g of bitter vegetable polysaccharide, wherein the polysaccharide has a purity of 62%.

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Abstract

This invention discloses a method of extracting dissolvable polysaccharide from common sowthistle herb. drying and crushing fresh common sowthistle herb After it is cleaned and open-air dried; then mixing with distilled water according to proportion by weight is 1:15-1:40, hot diffused 4-8 hours at 80DEG C-100DEG C, filtering after cooling; filter liquor is centrifuged , getting liquid phase and sediment; At last liquid phase and alcohol whose density is 80% is mixed according to proportion by weight is 1:5-1:10, getting eluant after churned, repeating 2-4 times like this; When eluant is vacuum dried, it is completed.

Description

technical field [0001] The invention relates to a method for extracting soluble polysaccharides, in particular to a method for extracting soluble polysaccharides from bitter cabbage of Patrinaceae. Background technique [0002] Bitter cabbage, scientific name Patrinia, is a plant of Patrinaceae, which is a traditional Chinese medicine and wild vegetable in my country. It has been eaten by many people. Many bitter vegetable planting bases have been established in Jiangsu and Zhejiang. It is reported that it has the effect of treating dysentery and diarrhea. Many studies have shown that plant polysaccharides have anti-tumor, anti-virus, anti-aging, hypoglycemic, hypolipidemic and Anticoagulation and other biological activities. However, the biological activities of bitter vegetable polysaccharides, such as immune regulation, anti-tumor, hypoglycemic, and hypolipidemic, need to be further studied. There are many reports on the efficacy research and chemical composition of bit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 朱加进施佳慧程俊文
Owner ZHEJIANG UNIV