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Apparatus for moving particles from a first fluid to a second fluid

A first-fluid, fluid technology, applied in the preparation of test samples, fluid controllers, containers for laboratory use, etc., can solve the speed limitation, lack of perfect automation, and little potential for the development of rapid cell monitoring systems, etc. problem, to minimize the loss of operation

Inactive Publication Date: 2008-11-19
THE SEC OF STATE FOR DEFENCE IN HER BRITANNIC MAJESTYS GOVERNMENT OF THE UK OF GREAT BRITAIN & NORTHERN IRELAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method itself requires manual operation, so the speed of processing such samples by the above method is limited
Although automation is possible, there is currently no well-established path to automation and little potential for the development of rapid cell monitoring systems

Method used

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  • Apparatus for moving particles from a first fluid to a second fluid
  • Apparatus for moving particles from a first fluid to a second fluid
  • Apparatus for moving particles from a first fluid to a second fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The first preferred equipment

[0049] First Fluid / First Inlet: Degassed Water

[0050] Second fluid / second inlet: Yeast cell suspension (rehydrated dry, Boots, Nottingham, UK, 1×10 8 ml -1 ).

[0051] The total volumetric flow rate was controlled at 4.65 ml / min by three pumps (Gilson Mini-puls 3) and the tubing setup previously described by J.J. Hawkes and W.T. Coakley in Sensors and Actuators B, 2001, 75, 231-242. Set the first pump at the first outlet 16 (3.66ml / min), the second pump at the second outlet 18 (0.99ml / min), and the third pump at the second inlet 17 (0.56ml / min) . The flow of water from the reservoir (not shown) to the first inlet 15 (4.09ml / min) was controlled without a pump.

[0052] The Reynolds number in the channel 14 in this system is calculated to be 8.6, and assuming a parabolic flow path, it is calculated that the total flow rate between 12% of the total flow into the second inlet 17 and 88% of the total flow into the first inlet The dist...

Embodiment 2

[0060] Second preferred device

[0061] First Fluid / First Inlet: Degassed Water

[0062] Second fluid / second inlet: yeast cell suspension (1 x 10 6 ~2×10 8 ml min -1 ).

[0063] Yeast concentrations in all export samples were calculated by heamocytometer counts. Sodium fluorescein can be determined by absorbance at 485 nm (Shimadzu UV-2401PC spectrophotometer) by centrifugation of the samples and analysis of the supernatant.

[0064] The total volumetric flow rate was controlled at 10.2 ml / min by three pumps (Gilson Mini-puls 3) and the tubing setup described above. Set the first pump at the first outlet 16 (2.6ml / min), the second pump at the second outlet 18 (7.6ml / min), and the third pump at the second inlet 17 (1.7ml / min) . The flow of water (8.5ml / min) from the reservoir to the first inlet 15 is controlled without a pump.

[0065] The Reynolds number in the channel 14 in this system is calculated to be 37, and assuming a parabolic flow path, it is calculated that t...

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Abstract

There is disclosed apparatus for moving particles entrained in a first fluid to a second fluid, comprising a conduit, means providing for contacting laminar flow of each fluid within the conduit and means capable of generating a standing sound wave having a pressure node disposed within the conduit.

Description

technical field [0001] The present invention generally relates to apparatus and methods for moving particles between fluids. The present invention is particularly concerned with small-scale washing of microbial samples or the isolation of, for example, cells, spores and DNA, etc., but the invention is not limited thereto. Background technique [0002] Isolation and manipulation of microbial samples often requires one or more washing steps, which typically involve repeated centrifugation and resuspension of the sample. However, the above-mentioned method itself requires manual operation, so the speed of processing such samples by the above-mentioned method is limited. Although automation is possible, there is currently no well-established path to automation, and there is little potential for the development of rapid cell monitoring systems. [0003] There is therefore a need for an improved method that allows both cleaning of microbial samples and small-scale transfer betwe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J19/10B01D21/00B01L3/00G01N1/28G01N1/34G01N35/00
CPCB01J2219/00905B01L2400/0436B01L2200/0647B01L3/502761G01N1/34B01L2200/0636G01N2001/4094B01L3/502776G01N2035/00564B01J2219/00932B01L2400/0487B01D21/283
Inventor 杰里米·约翰·霍克斯威廉·特伦斯·科克利
Owner THE SEC OF STATE FOR DEFENCE IN HER BRITANNIC MAJESTYS GOVERNMENT OF THE UK OF GREAT BRITAIN & NORTHERN IRELAND