The invention relates to the field of bee breeding, in particular to a bee box with a queen wasp prisoning device. The bee box comprises a box body with an opening, supporting legs are arranged on theperiphery of a lower box wall, and an auxiliary cover, a honey box and a hive box are successively arranged between an upper box cover and the lower box wall; a vertically placed partition plate in aflat base tapered shape is in coupling connection with the inside of the honey box, and a vertically placed rectangular hive plate is in clearance fit with the inside of the hive box; an opening is formed in the center of a box cover, the queen wasp prisoning device penetrates through the opening and comprises a main rod, and the lower end of the main rod is slidably connected with a cylindricalsleeve, the sleeve is slidably connected with the main rod, and a transverse horizontal plate is placed below the sleeve; at least five cage bars surrounding the cylindrical sleeve to form a round matrix are welded with the sleeve and the transverse horizontal plate, and the distance between any two adjacent cage bars is same. The purpose is that by utilizing the cage bars, the sleeve and the transverse horizontal plate, a sliding block connecting rod mechanism is formed, the activity range of the queen wasp in the queen wasp prisoning device is gradually expanded, and therefore the problem that due to the fact that the queen bee is released, the queen wasp is in a rage state to run around, so that worker bees are irritated to surround the queen wasp is solved.