Reagent for fast diagnosing mammitis of ox, sheep, pig, preparation process and application thereof

Abstract

The invention discloses a rapid diagnosis reagent for mastitis of cattle, sheep and pig, and the components and the weight per cent is: Sodium dodecyl benzene sulfonate 2.5%-thirty%, sodium hydroxide 1%-15.5%, alternate sodium nitride 0.05%-0.5%, bromocresol purple 1%-10%, and water 45%-95%. The invention also discloses the preparation method for the reagent that is making up water solution with sodium hydroxide, alternate sodium nitride and adding sodium dodecylsulfate, bromocresol purple according to the ratio, whisking and mixing to make it fully dissolving. The invention has fast speed, low cost, high accuracy, repetitiveness and good stability. It is easy to operate, and has broad application range.

Description

technical field [0001] The invention relates to a diagnostic reagent, in particular to a rapid diagnostic reagent for cattle, sheep and pig mastitis, a preparation method and application thereof. Background technique [0002] Mastitis is the response of mammals (mainly referring to cattle, sheep, pigs, and emphatically referring to cows) to damage to the milk-secreting tissues inside their udders. The invasion of pathogenic microorganisms and the toxins they secrete are the main causes of mastitis. According to research reports, the incidence of mastitis in foreign dairy cows is about 40%-70%, and the incidence of mastitis in domestic cows is about 50%-85% (Zhengzhou City Dairy Cow Mastitis Investigation Group, 1988). 10%-30%, the incidence of recessive mastitis is about 40%-70% (Jiang Wucheng, 1993). After the occurrence of mastitis in animals, it will lead to huge losses such as waste of milk, a significant decrease in milk production, and a decrease in milk quality. Acc...

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Problems solved by technology

The price of the instrument ranges from several thousand yuan to tens of thousands of yuan, which is beyond the purchasing power of individual farmers, and its operation requires certain technical and power supply conditions, and it cannot be tested on the spot next to cattle, sheep and pigs, which limits its promotion. ,application

In the 1980s, dairy research institutions in Lanzhou and Beijing imitated CMT and developed their own laboratory diagnostic methods for dairy cow mastitis. The main reagents were benzene sulfate and thymol blue, but they have not been popularized and applied in China for unknown reasons; In 2005, researchers from Agriculture and Forestry University also applied for the patent of "a new reagent for the diagnosis of recessive mastitis". According to the published components, there are many types of components. Because there are no molded products on the market, the effect is unknown.

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