Novel compound neopuerarin A and its separating method
A technology of puerarin and separation method, applied in chemical instruments and methods, plant/algae/fungus/moss components, sugar derivatives, etc.
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Embodiment 1
[0083] Compound puerarin A, its structural formula is as follows:
[0084]
[0085] The separation method is as follows: select 2000 g of kudzu root decoction pieces made from the dried root of Pueraria mirifica, extract once with 10 times the volume of 50-95% ethanol, and filter for 2 hours each time; combine the filtrates and concentrate under reduced pressure to After 800ml, dilute to 3500ml with water, pass through the AB-8 macroporous adsorption resin column, elute with water until the effluent is nearly colorless, then elute with 30-70% ethanol, wash until the ethanol eluate is nearly colorless, Combine the above-mentioned ethanol eluents, concentrate under reduced pressure, and then dry them in a vacuum oven at 50-70°C to obtain 300 g of dry extract;
[0086] The dry extract 300g is separated by silica gel column chromatography, i.e. chromatographic separation I, chloroform-methanol mixed solvent 100:0 → 20:1 → 10:1 → 4:1 gradient elution, collect in portions, collec...
Embodiment 2
[0088] Compound puerarin A, its structural formula is as follows:
[0089]
[0090] The separation method is as follows: select 3000 g of kudzu root decoction pieces made from the dried root of the leguminous plant Pueraria mirifica, extract twice with 8 times the volume of 50-95% ethanol, and filter for 1.5 hours each time; combine the filtrates and concentrate under reduced pressure to After 1000ml, dilute to 3000ml with water, pass through the AB-8 macroporous adsorption resin column, elute with water until the effluent is nearly colorless, then elute with 30-70% ethanol, and wash until the ethanol eluate is nearly colorless, Combine the above-mentioned ethanol eluents, concentrate under reduced pressure and dry in a vacuum oven at 50-70°C to obtain 400 g of dry extract;
[0091] The dry extract 400g is separated by silica gel column chromatography, that is, chromatographic separation I, chloroform-methanol mixed solvent 100:0 → 20:1 → 10:1 → 4:1 gradient elution, collec...
Embodiment 3
[0093] Compound puerarin A, its structural formula is as follows:
[0094]
[0095] The separation method is as follows: select 4000 g of Pueraria decoction pieces made from the dried root of Pueraria mirifica, extract 3 times with 6 times the volume of 50-95% ethanol, and filter for 0.5 hours each time; combine the filtrates and concentrate under reduced pressure to After 1200ml, dilute to 2500ml with water, pass through AB-8 macroporous adsorption resin column, elute with water until the effluent is nearly colorless, then elute with 30-70% ethanol, and wash until the ethanol eluate is nearly colorless, Combine the above ethanol eluents, concentrate under reduced pressure, and then dry them in a vacuum oven at 50-70°C to obtain 500 g of dry extract;
[0096] The dry extract 500g is separated by silica gel column chromatography, that is, chromatographic separation I, chloroform-methanol mixed solvent 100:0 → 20:1 → 10:1 → 4:1 gradient elution, collect in portions, collect o...
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