Unlock instant, AI-driven research and patent intelligence for your innovation.

Culture method of external tissue and its fatiscent carrier

A culture method and technology for bone tissue, which can be used in tissue culture, tissue cell/virus culture devices, biochemical equipment and methods, etc., and can solve the problem of uneven growth of external bone tissue cells.

Inactive Publication Date: 2009-08-26
IND TECH RES INST
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overcome the dedifferentiation phenomenon of bone tissue in two-dimensional plane culture in vitro, or the uneven growth of bone tissue cells inside and outside the base material, and achieve the purpose of effectively culturing bone tissue in vitro

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture method of external tissue and its fatiscent carrier
  • Culture method of external tissue and its fatiscent carrier
  • Culture method of external tissue and its fatiscent carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] A method for culturing in vitro cartilage tissue:

[0048] (1) Preparation of a bioabsorbable porous carrier 10 having a hollow cavity 2:

[0049] The decomposable polymer material used in this example is a PLGA polymer (molecular weight is about 200,000) prepared by ring-opening polymerization. The blocky PLGA polymer material is crushed in a pulverizer, and the crushed particles are sieved through a 60-80 mesh screen to obtain polymer particles with a particle size of 177-250 μm; the selected ones are added to the substrate to create holes. The water-soluble material of the structure is sodium chloride particles with a particle size of about 250 μm; the selected organic solvent for dissolving polymer particles is acetone.

[0050] PLGA polymer particles and sodium chloride particles are uniformly mixed by stirring according to the weight ratio of 10 / 90; the mixed PLGA polymer particles and sodium chloride particles are poured into a circle with a suction device at th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to View More

Abstract

The present invention relates to a culture method for external tissue and its polycellular carrier. It is mainly characterized by that after the tissue block is partially dissolved by ferment, it can be placed in the hollow cavity of the polycellular carrier with hollow cavity to make the tissue grow toward the pores of peripheral polycellular carrier from interior in the mode of three-dimensional space and directly proliferate new tissue in the form of tissue. Said invention has the function of that uses smallest volume tissue quantity to culture required tissue in vitro in shortest time.

Description

technical field [0001] The invention relates to a method for culturing bone tissue in vitro and its porous carrier, which is to put the extracted bone tissue block into the hollow porous decomposition carrier, and supply nutrients so that the bone tissue expands to the surrounding in a three-dimensional manner. The pores of the porous substrate grow to obtain new bone tissue. Background technique [0002] As we all know, when human tissues or organs are damaged beyond repair due to factors such as accidental injury or disease and aging, it often results in the disability of the patient's limbs or threatens the life of the patient, further causing a heavy burden and loss to the family and society. Therefore, seeking a suitable repairing replacement tissue or organ is the goal that researchers are constantly working on. [0003] In recent years, with the advancement of biotechnology, biological and medical materials and tissue cell culture techniques have been combined with e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08C12M3/00
Inventor 廖俊仁林玉如陈进富张根源江淑芳
Owner IND TECH RES INST
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com