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Monoclonal antibody, gene encoding the same, medicinal composition and diagnostic reagent

A monoclonal antibody and composition technology, applied in the direction of drug combination, antibody, anti-tumor drug, etc., can solve the problem of not knowing vimentin

Inactive Publication Date: 2009-09-23
MITSUBISHI TANABE PHARMA CORP +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Moreover, it is known that vimentin is not expressed in normal epithelial cells, but it is found that vimentin is highly expressed in the cytoplasm of some poorly differentiated tumor cells such as lung adenocarcinoma, gastric cancer, endometrial cancer or germ cell carcinoma (Molecular Cell Biology (Mol Cell Biol.), 1987, Vol. 7, No. 11, pp. 3908-3915)
However, the phenomenon that vimentin acts as an antigenic protein is not yet known

Method used

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  • Monoclonal antibody, gene encoding the same, medicinal composition and diagnostic reagent
  • Monoclonal antibody, gene encoding the same, medicinal composition and diagnostic reagent
  • Monoclonal antibody, gene encoding the same, medicinal composition and diagnostic reagent

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Embodiment

[0084] Hereinafter, the present invention will be described in more detail with reference to Examples. However, the present invention is not limited to these examples without departing from the scope of the present invention.

[0085] (1) Preparation of hybridomas by cell fusion of cancer-infiltrating lymphocytes obtained from cancer patients and mouse myeloma cells

[0086] (1)-1: Preparation of lymphocytes

[0087] Tumor tissues resected from lung cancer patients were cut into small pieces with a scalpel, then sufficiently suspended in medium A (RPMI1640+50 μg / ml gentamicin sulfate), and the medium was collected (referred to as medium (I)) . The small pieces of tumor tissue were further minced into fine pieces with a razor blade, and the cells were then dispersed in fresh Medium A by pipetting. These cell suspensions were centrifuged at 1,000 rpm for 5 minutes, and the supernatant (referred to as supernatant (II)) was collected, the medium (I) and the supernatant (II) wer...

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Abstract

The present invention relates to novel human monoclonal antibodies that specifically recognize cancer cells such as non-small cell lung cancer, pancreatic cancer, and gastric cancer, which are produced by hybridomas obtained from lymphoid tissues obtained from cancer patients. Cells were obtained by fusion with mouse myeloma cells. The present invention also relates to anticancer drugs which can be obtained by using antibodies alone or by anchoring antibodies to the surface of liposomes in which toxins or anticancer drugs are encapsulated. More specifically, the present invention relates to anticancer drugs obtained by using antibodies alone or by anchoring antibodies to the surface of liposomes in which toxins or anticancer drugs are encapsulated, wherein the The heavy chain variable region of the antibody comprises the amino acid sequence set forth in SEQ ID NO:115, and the light chain variable region thereof comprises the amino acid sequence set forth in SEQ ID NO:117.

Description

technical field [0001] The present invention relates to novel monoclonal antibodies useful for the diagnosis and treatment of cancer, DNA encoding the monoclonal antibodies, hybridomas producing the antibodies, pharmaceutical compositions and diagnostic reagents containing the antibodies. Background technique [0002] In the field of cancer treatment, directed therapy against a specific type of cancer cells has been currently being studied for the treatment of solid cancers, for which no therapeutic agents have shown effective effects. In the targeted therapy, monoclonal antibodies that specifically recognize cancer cells are effective. However, the use of mouse monoclonal antibodies has some problems, such as difficulty in repeated administration due to side effects such as allergic reactions caused by immune responses (Proc. Natl. Acad. U. SA, Vol. 86 4220 pages, 1989). [0003] In order to overcome the problems, attempts have been made to obtain monoclonal antibodies wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/00C07K16/32C12N5/00C12P21/08A61K39/395A61K9/127A61P35/00G01N33/574
Inventor 细川齐子青木真彦平川容子伊丹清马梅木裕惠才川义朗熊井浩一郎福田和正
Owner MITSUBISHI TANABE PHARMA CORP
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