Automatic inspection of mouse exercising studying memory abilities after being stressed

Abstract

An automatic test method for the movement, learning and memory powers of mouse after stressed includes such steps as fixing a mouse to a experiment table with annular screen and camera, aligning the camera with the eyeball of mouse, focusing to obtain a clear eyeball image, rotating said annular screen to capture the image information of eyeball, inputting it to a computing unit, storing and processing the information to obtain the boundary information and central position of pupil, coinciding the circular boundary curve with the created pupil boundary, repeatedly recording the Nth central position of pupil and the position of a point on created pupil boundary at time intervals, and calculating the variation. Speed and amplitude of pupil within time T is calculated.

Description

technical field [0001] The invention belongs to the automatic observation method of medical animal experiment behavior, and in particular relates to a computer image acquisition and analysis method and a method for automatic detection of motor learning and memory ability of mice after stress by using electric pulse trigger recording technology. Background technique [0002] During the war years, most of the combat effectiveness of soldiers was achieved through learning and memory. Such learning and memory can be divided into two categories: one is the spatial learning and memory ability based on the hippocampus, such as the ability to quickly distinguish directions on the battlefield, The learning and memory of maps and terrain, and the learning and memory of the field environment; one is the motor learning and memory ability based on the cerebellum, such as the motor coordination ability during fast marching, and the visual learning and memory coordination ability when quick...

AI Technical Summary

Problems solved by technology

[0006] There are big flaws in this measurement method: ① figure 1 As shown, what is actually measured is the position of the center of the rectangular strip passing through the center of the mouse pupil loaded by the position analyzer. The mouse's struggle, eyelid closing, and pupil narrowing will all lead to measurement errors, and the mouse HOKR detection system High precision is required, and a small measurement error will lead to a huge change in the result; ②In order to avoid this measurement error, the experimenter needs to adjust the position of the rectangular strip at any time through the position analyzer during the observation process, which will add a lot of extra workload and introduces another factor of subjective measurement error

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