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Chinese herb discrimination

A technology of capillary electrophoresis and electrochemical analysis, which is applied in the direction of material analysis, material analysis, and measuring devices through electromagnetic means, which can solve the problems of complex, unclear, and easy to cause pollution in traditional Chinese medicine

Inactive Publication Date: 2009-12-16
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When HPLC analyzes the active ingredients of traditional Chinese medicine, its advantages are high column efficiency, good selectivity, and wide application range, etc., but there are also the following disadvantages: (1) The ingredients of traditional Chinese medicine are complex and unclear, which increases the difficulty of sample pretreatment , and it is easy to cause pollution and affect the life of the column; (2) The column efficiency of each column is actually less than 10,000 trays; (3) The amount of solvent consumed is large; (4) The analysis time is generally longer
But so far, there are no literature and patent reports on CE-ED fingerprint identification of Chinese herbal medicines

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Quantitative detection and fingerprint identification analysis of different parts of Aristolochia plant

[0038] The fruits, leaves and roots of the plant Aristolochia are all herbs. They are called Aristolochia, Aristolochia and Aristolochia.

[0039] (1) The specific method for preparing buffer solution is:

[0040] Use 1.0mol L of phosphate buffer solution (PBS) with pH values ​​of 9.5, 10.0, 10.5 and 11.0 -1 Adjusted by NaOH, pH value: 7.0, 8.0 and 9.0 buffer solutions are prepared by directly mixing Na 2 HPO 4 And NaH 2 PO 4 The aqueous solution is sufficient.

[0041] (2) The preparation of AAs standard solution and its cyclic voltammetry experiment are as follows:

[0042] Preparation of AAs standard solution: containing 7.6×10 -4 mol L -1 AA-I and 2.0×10 -3 mol L -1The mixed solution of AA-II is prepared with methanol and stored in a refrigerator at 4°C, and diluted with secondary water to the required concentration before use.

[0043] Cyclic voltammetry...

Embodiment 2

[0064] Example 2: Quantitative detection and fingerprint identification analysis of three kinds of Mutong

[0065] Three kinds of herbs: Guanmutong, Chuanmutong, and Mutong are often confused by people because of their similar appearance, and they are all called "mutong" in Chinese. However, they belong to different kinds of plants. In practical applications, people often misuse the nephrotoxic and carcinogenic Guanmutong instead of the non-toxic Chuanmutong and Mutong. The main active ingredient of Guanmutong with nephrotoxicity and carcinogenicity is AAs. Therefore, the quantitative detection of AAs and fingerprint identification of the three types of Guanmutong are very beneficial for the safe use of these herbs to prepare traditional Chinese medicine.

[0066] (1) The preparation of buffer solution, the specific method is:

[0067] Use 1.0 mol L of phosphate buffer solution (PBS) with pH values ​​of 9.5, 10.0, 10.5 and 11.0 -1 Adjusted by NaOH, pH value: 7.0, 8.0 and 9.0 buffe...

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PUM

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Abstract

A fingerprinting process for discriminating Chinese-medicinal material by capillary electrophoresis and electrochemical analysis method includes such steps as preparing buffer solution, preparing the standard solution of the active component, cyclic V-A test, optimizing the test conditions, preparing the solution of Chinese-medicinal material specimen, obtaining its CE-ED fingerprint, comparing the peaks of active components in standard solution and specimen solution to determine the content of active component in specimen, and comparing the contents of active components and fingerprints.

Description

Technical field [0001] The invention relates to a method for determining the content of aristolochic acid I and aristolochic acid II in Chinese herbal medicines by capillary electrophoresis-electrochemical analysis technology. Background technique [0002] The application of Chinese herbal medicine has a history of thousands of years in our country and is the crystallization of the excellent culture of the Chinese nation. The analysis of Chinese herbal medicine is very important, but it is also very complicated. First, different types of herbs contain different active ingredients; secondly, the active ingredients of the same herb are also affected by factors such as soil, climate and growth stage. Even for the same herb, the content of active ingredients in different parts is also different; , Chinese herbal medicine also has certain side effects. Therefore, how to quickly, efficiently and conveniently perform qualitative and quantitative analysis of active ingredients in Chinese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447A61K36/00
Inventor 由天艳周晓光
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES