Unimolecular tracing method in low viscosity solution

Abstract

The invention relates to single molecule tracing method in low viscosity solution. It uses dye marked molecule as tracing object, dehydrated agarose gel to decorate slide, processes fluorescence imaging for the single molecule in the solution between the cover glass and agarose gel. It includes the following steps: forming agarose gel film on the slide; covering the cover glass; sending to obverse by the microscope to do single molecule fluorescence imaging. The method of invention can realize dynamical real time monitoring, has low cost, simple and fast operation.

Description

Technical field: [0001] The invention mainly relates to a single-molecule tracing method in a low-viscosity solution. Background technique: [0002] So far, what traditional analytical chemistry has measured is actually the ensemble average of a large number of molecules, and the statistical results of this ensemble can no longer meet the analysis requirements for complex and non-homogeneous biological systems. In recent years, an analytical technique that targets single molecules has emerged. Detection technology at the single-molecule level has become a powerful supplement to traditional ensemble research methods, based on individual molecules, to study macroscopic systems. Compared with the ensemble method which only provides the average properties of the system, single-molecule detection technology can detect the distribution and time trajectory information of physical quantities, and can study the fluctuation of the system in equilibrium and the reaction pathway in non...

AI Technical Summary

Problems solved by technology

[0008] Although these instruments can track single molecules to a certain extent, they are costly, complicated, and have unsatisfactory signal-to-noise ratios. They cannot track multiple single molecules reacting at the same time, which will interfere with the reaction process.

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