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Microreactor for genetic test

A technology of microreactor and gene, which is applied in the direction of specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, laboratory container, etc., which can solve the problem of increased production cost and insufficient reproducibility, etc. problem, to achieve the effect of efficient adsorption

Inactive Publication Date: 2007-10-03
KONICA MINOLTA MEDICAL & GRAPHICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A DNA chip immobilized with multiple DNA fragments at a high density has problems such as insufficient information content, increased production cost, detection accuracy, and reproducibility.

Method used

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  • Microreactor for genetic test
  • Microreactor for genetic test
  • Microreactor for genetic test

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Experimental program
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Embodiment Construction

[0013]

The microreactor for genetic testing according to the present invention is characterized in that the reaction part is a fine flow in the front starting from the confluence part where the treatment solution for the pretreatment of the sample to be tested and the reagent stored in the amplification reagent storage part merge. road composition,

A control device is provided for switching the feeding direction of the combined liquid of the respective liquids sent to the reaction part, and repeatedly moving the combined liquid back and forth in the reaction part.

[0014]

In the above-mentioned invention, since the confluence of the treatment liquid and the reagent, which is pretreated in the carrier filling part, switches the direction of liquid delivery in the reaction part, moves back and forth, and performs the amplification reaction at the same time, so the probability of encountering the bacterial gene and the reagent is increased. , which increases the reaction sp...

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Abstract

The invention provides gene-inspecting micro-reactor for detecting a bacterial cell wherein flexibility and high sensitivity are secured, low cost by a disposable type is realized, highly accurate detection is possible in a simple structure, and preliminary processing suitable for amplification reaction can be done for specimen efficiently and rapidly. After lysing a bacterial cell in the specimen by bacteriolysis reagent stored in bacteriolysis reagent storage section 3, and after adsorbing bacterial genes on carriers filled in carrier filling section 4, there is conducted preliminary processing for washing and detecting bacterial genes. Further, there are provided a control means that switches the direction of liquid-feeding for a liquid containing bacterial genes fed to carrier filling section, a check valve, amplified reagent storage section and a cooling means such as Pertier element that cools reagent mixing section.

Description

technical field [0001] The present invention relates to a method for carrying out gene amplification by transporting a sample to be tested injected from a sample storage part to be tested or a treatment solution pretreated on the sample to be tested, and a reagent stored in the amplification reagent storage part to a reaction part. After the reaction, a microreactor for genetic testing in which the operation of detecting the gene amplification reaction with the detection part is carried out in the chip in which the above-mentioned parts are connected through a fine flow path, especially a microreactor suitable for detecting bacteria from the sample to be tested . Background technique [0002] In recent years, devices and components (such as pumps, valves, flow paths, sensors, etc.) that will be used for existing sample preparation, chemical analysis, chemical synthesis, etc. have been developed by driving microreactor technology and ultrafine processing technology Minia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/34G01N33/50
CPCB01L3/50273B01L3/502753B01L2200/027B01L2200/10B01L2200/16B01L2300/0816B01L2300/087B01L2400/0439B01L2400/0605F04B43/046G01N35/1095G01N2035/1034
Inventor 中岛彰久上田荣一山东康博东野楠石田畅久
Owner KONICA MINOLTA MEDICAL & GRAPHICS INC
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