Biological extraction process of glycyrrhizic acid

Abstract

The present invention discloses biological extraction process for licorice, intermediate and end product made from main chemical components thereof. The process includes the following steps: crushing licorice into fine powder, enzymolysis, filtering and fermentation for full dissociation, centrifuging the fermented liquid to eliminate thallus, deactivating, chemically depositing to separate out coarse glycyrrhetic acid product, and purifying to obtain purified glycyrrhetic acid product. The coarse glycyrrhetic acid product and the purified glycyrrhetic acid product may be prepared into various kinds of medicine preparation. The present invention has the advantages of high yield of effective components, reduced waste slag, low cost, low power consumption and simple technique route, fully avoids environment pollution caused by excessive use of strong acid, strong base and harmful solvent, overcomes effective chemical component racemization, isomerization and structure rearrangement caused by vigrous chemical reaction.

Description

technical field [0001] The present invention relates to the technical field of production of medicines, food, daily chemicals, cosmetic additives, veterinary medicine and feed additives, relates to a biological extraction technology for chemical components of traditional Chinese medicine and its application, specifically, the application of enzyme engineering and fermentation engineering for The process of the core technology extracts glycyrrhizic acid, its main chemical component, from licorice, and makes it into intermediate raw materials or end products. Background technique [0002] Glycyrrhiza (Glycyrrhiza) is a perennial plant belonging to the leguminous family, with many species. It is mainly distributed in Northwest my country, North China, Central China and Northeast China. It has strong vitality and is one of the important plant resources in arid and semi-arid areas. Licorice has been recognized as a dual-purpose plant in my country. Currently, it is widely used in...

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Problems solved by technology

On the other hand, as an important species of sand-fixing plants, licorice is also of great significance to control deserts and prevent land desertification. Therefore, the state has issued regulations many times to prohibit the indiscriminate digging of wild licorice in the place of origin, which has exacerbated the acquisition and production of wild licorice. Utilize the pressure of licorice resources; although licorice has been cultivated artificially, it is still far from meeting the strong demand of domestic and foreign markets

[0004] From the perspective of the extraction technology of the main chemical components of licorice, the current conventional technology almost all involves the application of strong acid, strong alkali and various organic extractants, and there are a series of disadvantages as follows: ① Excessive extraction materials and equipment increase production costs; ②Excessively produced chemical agent extraction waste liquid seriously pollutes the environment; ③Conventional technology cannot effectively remove the structural barrier effect of a large amount of lignin and cellulose contained in rhizome traditional Chinese medicines on pharmacodynamic components, resulting in partial dissolution and yield of active components. Low, a large amount of dregs are discarded, which is a serious waste of resources and pollutes the environment; ④The severe chemical reaction conditions of strong acid, strong alkali and other extraction solvents cause problems such as racemization, isomerization and structural rearrangement of effective chemical components, which significantly affect Stability, purity and pharmaceutical effects of active ingredients; ⑤ Inorganic and organic extraction solvents have strong pharmacological effects and / or explosiveness, corrosion, etc., which pose a safety threat to the working environment and human health

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