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Plant miticide derived from artemisia annul l and method for preparing the same

A plant-based, Artemisia annua technology, applied in the field of plant-based acaricides

Inactive Publication Date: 2008-02-06
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In the prior art, no one has considered using and / or confirming the use and / or confirmation of the extract of Artemisia annua of the Compositae plant as an acaricide, and there are only reports on the insecticidal activity of the Artemisia annua of the Compositae plant in foreign countries

Method used

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  • Plant miticide derived from artemisia annul l and method for preparing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The selection of embodiment 1 Artemisia annua plant parts

[0054] Differences in Acaricidal Activity of Different Plant Parts of Artemisia annua in June

[0055] With different solvent extracts of 5 mg / ml Artemisia annua in June, the contact toxicity of different plant parts to Tetranychus cinnabarinus was measured under laboratory conditions, and the results are shown in Table 3:

[0056] Table 3 The biological activity of different solvent extracts of Artemisia annua in June to Tetranychus cinnabarinus (5mg / ml, 48h)

[0057]

different solvent extracts

Corrected mortality % (mean ± standard error)

root

stem

leaf

Petroleum ether I

Petroleum ether II

ethanol

acetone

water

Petroleum ether II sequence

ethanol sequence

Acetone sequence

water order

36.54±1.11cdBCD

43.64±1.82bcBC

45.10±2.26bBC

66.00±3.05aA

23.27±3.05eD

30.82±2.52dCD

46.06±1.6...

example 2

[0060] The acaricidal activity comparison of example 2 Artemisia annua leaves acetone extract

[0061] The bioassay results of more than 100 kinds of extracts of Artemisia annua in April, May, June, July and September showed that the biological activity of the parallel extracts of A. Significant differences exist. For further research on the activity difference of five kinds of extracts, the toxicity regression analysis of five kinds of extracts to Tetranychus cinnabarinus was measured, and the lethal middle time (Medianlethal time, LT 50 ). The results are listed in Table 4 and Table 5.

[0062] Table 4 The toxicity regression analysis of the acetone extract of Artemisia annua leaves to Tetranychus cinnabarinus in five months (48h)

[0063]

[0064] As can be seen from Table 4, the LC of the acetone parallel extracts of leaves of Artemisia annua in different months to Tetranychus cinnabarinus 50 Varies with the season of collection, LC in April, May, June, July...

example 3

[0068] The comparison of the acaricidal activity of the different components obtained by the column chromatography of the wormwood leaf acetone extract of example 3

[0069] The acaricidal activity of different components obtained by column chromatography of the acetone extract of Artemisia annua leaves was determined by the biological activity tracking method, and the results are shown in Table 6.

[0070] It can be seen from Table 6 that among the 13 finally separated components, component 12 had the highest acaricidal activity, and the corrected mortality rate within 48 hours reached 99.20%, and its 95% CI was 96.24-102.34. And 6 times, the average corrected mortality within 48h reached 82.52%, 89.74%, respectively, and its 95% CI were 81.79 ~ 83.24, 85.08 ~ 94.41mg / ml. It can be seen that the acaricidal active substance of Artemisia annua mainly exists in the acetone extract, and the acetone extract of the leaves has the highest content.

[0071] Table 6 Acaricidal activi...

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Abstract

The invention relates to a plant source acaricide made from the artemisia annua L. The invention has the artemisia annua L. extract as the main ingredient, together with additional synergist, emulsifying agent and dispersing agent, wherein the percentage by weight of the artemisia annua L. extract which consists of terpenoids, flavonoids and volatile oil compounds is 1 to 15 percent of the mixture. The acaricide is capable of being used to control phytophagous mites harming vegetables, oranges and apples and wheat mites hidden in food, in particular to control carmine spider mite, citrus red mites and copra mites; therefore, the invention has the advantages of high efficiency, low toxicity and no environmental pollution, and does not harm the quality of agricultural products. In addition, the invention also relates to the method of preparation of the plant source acaricide.

Description

technical field [0001] The invention relates to a botanical acaricide, in particular to a botanical acaricide mainly composed of the acaricidal active compound of the plant Artemisia annua and a preparation method thereof. Background of the invention [0002] Among the pests that humans need to control, mites are of special importance. According to the relationship with humans, harmful mites can be divided into agricultural mites, medical mites and storage mites. Agricultural mites mainly refer to herbivorous spider mites, whose damage is almost all crops and regions in the world. Tetranychuscinnabarinus is the most widely distributed and most serious agricultural pest in my country. The adults and nymphs of Panonychus citrus suck the leaves, tender stems and fruit juice of citrus. About 12-20 generations occur in one year, and the generations overlap. Winter and spring drought years occur early and frequently. Piercing the epidermis of the host leaves with the mouthpart...

Claims

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Application Information

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IPC IPC(8): A01N65/00A01P7/02A01N65/12
Inventor 丁伟张永强吴静尚德斌骆星丹李春华周宇杰
Owner SOUTHWEST UNIVERSITY
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