Compositions for induction of a therapeutic response
A composition and cell-inducing technology, which can be applied to medical preparations containing active ingredients, peptide/protein components, pharmaceutical formulations, etc., and can solve problems such as inconsistent successful results of immunotherapy
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Embodiment 3
[0176] Example 3 describes another study in which bioerodible polymer spheres containing one or more biological agents were prepared. The bioerodible microparticles were formed from DL-lactide-co-glycolide and contained a single agent GM-CSF, or a combination of agents G-CSF and RANTES. It will be appreciated that the preparation techniques described in Examples 2 and 3 can be used to entrap any desired single agent or combination of agents within porous or solid microparticles.
[0177] An alternative process for entraining a therapeutic agent in microparticles is to first add the therapeutic agent to a polymer solution, such as polyethylene oxide-polypropylene oxide- Copolymers of polyethylene oxide (Pluronic ), other water-soluble polymers, such as polyvinylpyrilidone (polyvinylpyrilidone), polyvinyl alcohol, or any other non-toxic water-soluble polymers, including biopolymers, such as collagen, cellulose, hyaluronate, etc. The aqueous solution of the therapeutic drug-p...
Embodiment 4
[0190] Example 4 describes the in vitro release of IL-12 from a depot comprising the biodegradable polymer, DL-lactide-co-glycolide. Briefly, IL-12 containing depots were placed in containers containing buffered saline and the release of IL-12 into the salt was measured as a function of time. After 11 days, 60% of IL-12 was released.
[0191] In use, as will be further described below, any of the depots described above are deposited, eg, as a small bolus, into, on, or onto the desired tissue site (see Examples 5 and 6). For example, a bolus of a drug reservoir can be deposited into the myocardium a few millimeters from the wall of the catheter where the deposition catheter is placed. The described drug depots are able to move freely through interstitial spaces in tissues. Each depot is primarily responsible for angiogenesis, arteriogenesis, and muscle cell regeneration, and as it moves through tissue, can facilitate the repetition of these processes at different locations. ...
Embodiment 5
[0199] Example 5 describes the use of a drug depot containing GM-CSF, MCP-1 and RANTES for cell and tissue regeneration after an ischemic event in rabbits, for example M-CSF, MCP-1 and RANTES have as chemoattractants activity of agents for cells involved in cell regeneration and as stimulants for differentiation and proliferation of these cells.
[0200] As another example, the drug depot can also be made to contain cell adhesion molecules, such as ICAM, to facilitate the adhesion of monocytes or other vascular-derived and arterial-derived cells to the tissue area. Cell adhesion molecules may be attached to the outer surface of one or more drug depots, or contained within said drug depots for release. Adhesion molecules appear on the plasma membrane of macrophages, fibroblasts and smooth muscle cells as ligands for adhesins and similar species. Adhesion molecules also attract macrophages to the drug depot and are able to physically bind to the drug depot to ensure macrophages...
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