Culture medium used for synthesizing conjugated linoleic acid and application method thereof

A technology of conjugated linoleic acid and medium, which is applied in the field of bioengineering, can solve the problems of insufficient conversion of CLA and affect the catalytic efficiency, and achieve the effect of avoiding consumption speed and improving efficiency

Inactive Publication Date: 2008-02-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the main problems in the production of CLA by microbial biotransformation are: LA is consumed as a carbon source and cannot be fully converted into CLA; LA cannot be dissolved in...

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0015] Embodiment 1, culture medium and preparation method

[0016] LA 100g

[0017] Absolute ethanol 100g

[0018] Glucose 200g

[0019] Soy flour 65g

[0020] Dissolve LA 100g in absolute ethanol 100g, then mix with glucose 200g, soybean powder 65g, dissolve thoroughly with water, obtain the culture medium of the present invention after filtering with a bacterial sieve.

Embodiment 2

[0021] Embodiment 2, the application of culture medium and the preparation method of CLA

[0022] The substratum described in embodiment 1 and the number of colonies are 1.5 * 10 8 Lactobacillus plantarum (Lactobacillus plantarum) 200ml per ml was mixed evenly, distilled to 1000ml with water, and cultured statically at 35°C for 13 hours.

[0023] The CLA content reaches 87.6g / ml, and the molar conversion rate is 87.6%.

[0024] Cultivated with the existing medium, the CLA content is less than 300mg / ml

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PUM

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Abstract

The invention discloses a substrate of the synthesis of conjugated linolic acid; the weigh proportion of each components of the culturation base is: 1 share fatty acid linolic acid, 1 share absolute ethyl alcohol, 2 shares glucoses, and 0.65 share soybean powder. The invention also discloses a preparation method of the substrate: 1 portion of fatty acid linolic acid is dissolved in 1 portion of absolute ethyl alcohol, mixed with 2 portions of glucoses and 0.65 portions of soybean powder, and the mixture is thoroughly dissolved by water and sieved by bacteria sieve, which can be used then. The invention further discloses an application method of the conjugated linolic acid synthesized by the culturation base: the substrate is added with high content glucoses, the lactobacillic acid mostly takes glocoses as a carbon source to prevent the lactobacillic acid from being decomposed, and still cells are adopts to synthesize CLA to cut down the carbon sources from being comsumed by cells; LA is dissolved in alcohol and added into the substrate, so as to homogenously distribute LA into the substrate and enhance the catalyzing effect.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a culture medium and a method for synthesizing conjugated linoleic acid by microorganisms using the culture medium. Background technique [0002] Conjugated linoleic acid (CLA) is a general term for 18-carbon conjugated dienoic acid geometric isomers derived from the essential fatty acid linoleic acid (Linoleic acid, LA) with conjugated unsaturated double bonds at different positions. . There are four main isomer positions, namely: C8, C10; C9, C11; C10, C12; C11, C13. And each isomer has four geometric isomers, namely: cc, ct, tc and tt, among which cis9, trans11 and trans10, cis12 two isomers have been proved to have strong physiological activity. At present, the most advantageous method of commercial production of CLA is the microbial biotransformation method. [0003] The culture of microorganisms is flexible and convenient. Many microorganisms can use their own linoleic acid...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12P7/64
Inventor 何国庆阮晖陈启和牛冬王凤平陈美龄张佳佳
Owner ZHEJIANG UNIV
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