Culture medium used for synthesizing conjugated linoleic acid and application method thereof

Abstract

The invention discloses a substrate of the synthesis of conjugated linolic acid; the weigh proportion of each components of the culturation base is: 1 share fatty acid linolic acid, 1 share absolute ethyl alcohol, 2 shares glucoses, and 0.65 share soybean powder. The invention also discloses a preparation method of the substrate: 1 portion of fatty acid linolic acid is dissolved in 1 portion of absolute ethyl alcohol, mixed with 2 portions of glucoses and 0.65 portions of soybean powder, and the mixture is thoroughly dissolved by water and sieved by bacteria sieve, which can be used then. The invention further discloses an application method of the conjugated linolic acid synthesized by the culturation base: the substrate is added with high content glucoses, the lactobacillic acid mostly takes glocoses as a carbon source to prevent the lactobacillic acid from being decomposed, and still cells are adopts to synthesize CLA to cut down the carbon sources from being comsumed by cells; LA is dissolved in alcohol and added into the substrate, so as to homogenously distribute LA into the substrate and enhance the catalyzing effect.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a culture medium and a method for synthesizing conjugated linoleic acid by microorganisms using the culture medium. Background technique [0002] Conjugated linoleic acid (CLA) is a general term for 18-carbon conjugated dienoic acid geometric isomers derived from the essential fatty acid linoleic acid (Linoleic acid, LA) with conjugated unsaturated double bonds at different positions. . There are four main isomer positions, namely: C8, C10; C9, C11; C10, C12; C11, C13. And each isomer has four geometric isomers, namely: cc, ct, tc and tt, among which cis9, trans11 and trans10, cis12 two isomers have been proved to have strong physiological activity. At present, the most advantageous method of commercial production of CLA is the microbial biotransformation method. [0003] The culture of microorganisms is flexible and convenient. Many microorganisms can use their own linoleic acid...

AI Technical Summary

Problems solved by technology

[0004] However, the main problems in the production of CLA by microbial biotransformation are: LA is consumed as a carbon source and cannot be fully converted into CLA; LA cannot be dissolved in water, which affects its catalytic efficiency.

Therefore, at present, the molar conversion rate of producing CLA through microbial biotransformation using LA as raw material in China is only about 40%.