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A method for simultaneously extracting phycoerythrin and agar from gelatinous seaweeds such as Astragalus

A technology of phycoerythrin and astragalus, which is applied in the application, food preparation, food science and other directions to achieve the effect of improving the utilization rate

Inactive Publication Date: 2011-11-30
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this species cannot spend summer and winter in the Yellow Sea and Bohai Sea, so it cannot effectively accumulate biomass.

Method used

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  • A method for simultaneously extracting phycoerythrin and agar from gelatinous seaweeds such as Astragalus
  • A method for simultaneously extracting phycoerythrin and agar from gelatinous seaweeds such as Astragalus
  • A method for simultaneously extracting phycoerythrin and agar from gelatinous seaweeds such as Astragalus

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Embodiment Construction

[0029] Below in conjunction with embodiment, the present invention is further described; Following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0030] This embodiment only takes the typical gelatin-containing seaweed—Asparagus asparagus as an example. Other gelatin-containing seaweeds can extract the contained alginate and related proteins according to this method, and this embodiment will not describe them one by one.

[0031] 1. Extraction and purification method of phycoerythrin

[0032] (1). Weigh 55 g wet weight of fresh asparagus and chop it up, add a small amount of distilled water overnight at 4°C to swell and lyse the cells.

[0033] (2). Centrifuge at 10,000 rpm and 4° C. for 10 minutes, collect all the supernatant, and obtain 340 ml of crude extract.

[0034] (3). According to the formula R-PE=155.8OD 498.5 -40.0OD 614 -10.5OD 651 , calculated to contain 66.630mg phycoerythrin in t...

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Abstract

The invention relates to a method for simultaneously extracting phycoerythrin and agar from asparagus and other gum-containing seaweeds in the technical field of seaweed biochemical industry. Precipitate with ammonium sulfate to obtain crude phycobiliprotein extract, extract the crude extract with Phenyl-Sepharose expansion column to obtain phycoerythrin product, and then use Q-Sepharose ion exchange column to purify phycoerythrin to obtain phycoerythrin Pure product; and then further extract the agar with the remaining asparagus residue after extracting phycoerythrin. The present invention can simultaneously obtain phycoerythrin and agar from asparagus, and the purity of the purified phycoerythrin is greater than 2.954, and the yield is 0.097mg / g fresh algae; the yield of the obtained agar is 2.31% (the algae is Wet weight), is suitable with the productive rate of directly extracting agar gum method, therefore can greatly improve the utilization rate of asparagus by the present invention.

Description

technical field [0001] The invention relates to the technical field of seaweed biochemical industry, in particular to a method for simultaneously extracting phycoerythrin and agar from asparagus and other gelatin-containing seaweeds. Background technique [0002] Asparagus (Gracilaria lemaneiformis) is an important cultivated species of Gracilaria, belonging to Rhodophyta, Rhodophyceae, Florideae, Gigartinales, Gracilaria Family (Gracilariaceae), Gracilaria (Gracilaria). Wild asparagus is mainly distributed in the intertidal zone along the coast of Shandong in my country. It has many branches and grows fast. The optimum growth temperature is 15-25°C. At present, asparagus has become the third largest cultivated seaweed after kelp and seaweed in my country. However, this species cannot spend summer and winter in the Yellow Sea and Bohai Sea area of ​​origin, so it cannot effectively accumulate biomass. For this reason, after realizing the cultivation of asparagus to the ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/28A23L1/337A23L1/305A23L1/0532A23J1/00A23L29/256A23L33/185
Inventor 王广策张开岳牛建峰
Owner TIANJIN UNIV OF SCI & TECH