The invention relates to tilapia mossambica feed and a preparation method thereof. The feed comprises protein source raw material, fat source raw material, sugar source raw material and auxiliary material, and is characterized by further comprising 15 percent of asparagus powder by weight percent, wherein the protein source raw material is selected from a group formed by rice bran, fish meal, peanut meal, bean pulp and rapeseed meal as well as a mixture of the rice bran, fish meal, peanut meal, bean pulp and rapeseed meal; the fat source raw material is selected from a group formed by fish oil and soya-bean oil as well as a mixture of the fish oil and soya-bean oil; the sugar source raw material is wheat middling; the auxiliary material is selected from a group formed by vitamin b complex, mixed mineral substances, soyabean lecithin powder, DL-methionine, monocalcium phosphate, choline chloride, ascorbic acid and sleeve-fish ointment as well as a mixture of the vitamin b complex, mixed mineral substances, soyabean lecithin powder, DL-methionine, monocalcium phosphate, choline chloride, ascorbic acid and sleeve-fish ointment; the feed comprises the following nutritional ingredients in percentage by weight: 35.9-36.8 percent of crude protein, 6.6-6.8 percent of crude fat, and the balance of carbohydrate, crude fibre, ash and moisture. According to the tilapia mossambica feed provided by the invention, the weight gain rate and survival rate of juvenile fish are higher, the use amount of fish meal can be reduced, and protein demand level of fingerling is reduced.