Check patentability & draft patents in minutes with Patsnap Eureka AI!

T-carrier

A vector, -CTGNN technology, applied in the field of vectors for cloning DNA PCR fragments, can solve problems such as low efficiency of PCR products

Inactive Publication Date: 2008-04-09
SHANTOU UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Directly using the blunt end ligation method to clone such PCR products is very inefficient. The solution is: firstly, eliminate the protruding bases at the 3′ end and turn the PCR product into blunt end DNA to improve the cloning efficiency, which will take a lot of time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • T-carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] 1. Design the following PCR primers according to the T-vector primer design idea,

[0028] forward primer: Ftv 5’-ACTGGTACCTGACAGTGAGTC

[0029] TAGCTGTTTCCTGTGTGAAAT-3', wherein GGTACC and GACAGTGAGTC are the recognition sites of restriction endonucleases KpnI and Eam1105I (AhdI) respectively.

[0030] reverse primer: Rtv: 5’-ACTGAGCTCTGACAGTGAGTC

[0031] GGCCTTTTTACGGTTCCTGG-3', wherein GAGCTC and GACAGTGAGTC are the recognition sites of restriction endonucleases SacI and Eam1105I (AhdI) respectively.

[0032] 2. Using pBluescript II SK (-) as a template, use the above two primers to amplify the DNA fragment (see 3 for the sequence). The amplified DNA fragment was separated, purified, digested with KpnI and SacI, and then ligated with pBluescript II SK(-) which had undergone the same digestion in advance.

[0033] 3. The DNA fragment sequence (pBS: 1199-816bp) amplified by the primers used in this example

[0034] tagct gtttcctgtg tgaaattgtt atccgctcac aattccaca...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention pertains to the molecular biology and biotechnology field and provides a T-carrier. The invention designs that the restriction sites of restriction enzyme Eam1105I or AhdI complements adhesive end with 3' A through restriction and is then inserted into a cloning carrier. The cloning carrier prepared according to the invention is characterized by high efficiency.

Description

technical field [0001] The invention relates to a carrier for cloning DNA PCR fragments, in particular to a T-vector fragment terminal nucleotide sequence. Background technique [0002] DNA cloning is an important technology in modern life science research. Cloning of specific genes is an important step in functional studies. The combination of PCR technology and DNA cloning greatly simplifies the steps of cloning a specific gene and shortens the time for cloning a specific gene, which not only greatly promotes the development of life sciences, but also has great application value in the fields of rapid diagnosis of diseases. [0003] PCR is an experimental method commonly used in biological and medical research to amplify DNA templates. Taq DNA Polymerase, which is commonly used in PCR, not only has the activity of replicating templates along the 5′ to 3′ direction at high temperature and prolonging DNA primers, but also can add one to several adenosine A to the 3′ end of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/63
Inventor 王子元刘文宽李伟杰谷福
Owner SHANTOU UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com