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Method for confirming warfarin dose of Chinese population individuals

A technology for determining the dosage and method of medication, which is applied in the direction of measuring devices, instruments, and material analysis through electromagnetic means. It can solve problems such as poor bleeding, incorrect estimation of drug dosage, and improper anticoagulation, so as to improve the effect and reduce bleeding. Chance of adverse event occurrence, effect of improving safety

Inactive Publication Date: 2008-05-21
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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AI Technical Summary

Problems solved by technology

However, warfarin anticoagulation therapy, especially in the initial stage of treatment, is prone to inappropriate anticoagulation, which leads to the occurrence of bleeding adverse events. Prevention of adverse events such as bleeding

Method used

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  • Method for confirming warfarin dose of Chinese population individuals
  • Method for confirming warfarin dose of Chinese population individuals

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Experimental program
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Embodiment

[0033] Embodiment: a method for determining the individual dosage of warfarin in the Chinese population, comprising the following steps:

[0034] (1) First, collect blood samples from the patient, then take 2ml of peripheral anticoagulant blood, use the Wizard(R) Genomic DNA Purification Kit, and extract DNA in strict accordance with the kit instructions;

[0035] (2) Perform PCR amplification on the extracted genomic DNA; PCR reaction system volume is 25 μl, genomic DNA 0.2 μg, 10XPCR buffer 2.5 μl, 2.5mmol / L dNTP 2μl, 25mmol / LMgcl 2 1.5 μl, primer P 1 10pmol, primer P 2 10pmol, Taq polymerase (5U / L) 0.15μl; PCR reaction conditions: 95°C pre-denaturation for 5min, 94°C for 30s, 59°C for 20s, 72°C for 30s, a total of 35 cycles, 72°C extension for 7min. VKORC1 gene forward primer is 5′-CCAGCAGGAGAGGGAAATA-3′, reverse primer is 5′-TTTGG-ACTACAGGTGCCT-3′; CYP2C9 gene forward primer is 5′-TGCACGAGGTCCAGAGATGC-3′, reverse primer is 5′-AAACATGGAGTTGCAGTGTAG -3', the primer des...

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Abstract

The invention discloses a method for confirming the warfarin quantity used for Chinese individual; the genome DNA of the patient is extracted for genotype detection and then the drug quantity of every patient is induced based on the age, weight and the genotype of VKORC1(-1639G more than A) and CYP2C9 to help the clinical doctor to use warfarin more rationally; in this way, INR proper level can be obtained in advance and the probability of bleeding and other adverse events; the safety for drug use and the treatment effect are promoted.

Description

technical field [0001] The invention relates to a method for determining the individual dosage of warfarin in Chinese population. Background technique [0002] Ischemic stroke is the leading cause of death in patients with atrial fibrillation (AF). Anticoagulant therapy is an important part of the treatment strategy for AF. Warfarin is currently the only one that can reduce the risk of stroke and embolism in patients with AF. Drugs that reduce mortality. However, warfarin anticoagulation therapy, especially in the initial stage of treatment, is prone to inappropriate anticoagulation, which leads to the occurrence of bleeding adverse events. Prevent the occurrence of adverse events such as bleeding. Because of risks such as bleeding and the cost of frequent INR monitoring, it has been estimated that about half of patients with atrial fibrillation are not anticoagulated with warfarin. Contents of the invention [0003] The purpose of the present invention is to provide a ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N27/447
Inventor 缪丽燕杨剑黄晨蓉沈振亚蒋文平
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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