Tobacco and tobacco product arsenic content determination method

A technology for tobacco products and determination methods, applied in the field of physical and chemical testing, can solve the problems of many types and dosages of digestion reagents, complex pretreatment, expensive instruments, etc., achieve good technology promotion value, shorten analysis time, and reduce types and dosages.

Active Publication Date: 2011-01-19
CHINA TOBACCO GUANGDONG IND
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The determination of arsenic in domestic tobacco mostly adopts atomic fluorescence method and ICP-MS method. The former is complicated in sample pretreatment, while the latter is expensive and difficult to popularize.
[0005] At present, there are relevant standards for the determination of arsenic in tobacco by graphite furnace atomic absorption using nickel nitrate as a chemical modifier. Compared with the traditional method, this method is much faster and more accurate, but the types and amounts of digestion reagents used are more, so it is not applicable Determination of Arsenic in Bulk Tobacco Samples

Method used

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  • Tobacco and tobacco product arsenic content determination method
  • Tobacco and tobacco product arsenic content determination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 working curve and determination of detection limit

[0021] 1. Main instruments and reagents

[0022] AAS800 atomic absorption spectrophotometer (PERKIN-ELMER company in the United States), equipped with THGA graphite tube and arsenic electrodeless discharge lamp; MWS-3+ microwave digestion system (BERGHOF company in Germany); H110 electronic balance (sensitivity 0.0001g, Germany SARTORIUS company).

[0023] 1000 μg / mL arsenic standard solution (provided by National Iron and Steel Research Institute); ammonium chlororhodium (analytical pure, Shanghai Jiushan Chemical Reagent Company); citric acid (analytical pure, Guangzhou Chemical Reagent Factory); 68% (mass fraction) nitric acid ( Superior grade, Guangzhou Donghong Chemical Factory); 30% (mass fraction) hydrogen peroxide (superior grade, Guangdong Guanghua Reagent Factory); ultrapure water (prepared by Milli-Q); tobacco leaves and cigarette samples.

[0024] Using the automatic dilution function of AAS...

Embodiment 2

[0027] The mensuration of arsenic content in the tobacco sample of embodiment 2

[0028] (1) Tobacco samples were pretreated and digested by nitric acid-hydrogen peroxide system microwave to prepare the test solution;

[0029] Weigh 0.2g of dried and pulverized 40-mesh smoke powder sample, put it in a 50mL microwave digestion tank, add 3mL of 68% nitric acid, let it stand at room temperature for 15min, add 1mL of 30% hydrogen peroxide, and cover with a polytetrafluoroethylene inner cover followed by microwave digestion. The digestion procedure is: heating up at 140°C for 2 minutes, holding for 10 minutes; heating up at 170°C for 1 minute, holding for 10 minutes; heating up for 1 minute at 200°C, holding for 10 minutes; cooling for 3 minutes at 100°C, holding for 7 minutes. After the digestion, cool to room temperature, open the tank, transfer the digestion solution to a 25mL volumetric flask, wash the digestion tank with 0.2% (volume fraction, the same below) nitric acid, inc...

Embodiment 3

[0035] Embodiment 3 relative phase difference, rate of recovery and repeatability experiment

[0036] Get 2 parallel samples according to the method described in Example 2, adopt the method of the present invention to measure its arsenic content respectively, the recorded arsenic content is respectively 0.44 and 0.46mg / kg, is 0.45mg / kg on average, and relative difference is 4.4%, Meet the requirements of GB / T5009.12-1996 relative difference ≤ 20%.

[0037] Take 3 different samples, all spiked with 5 μg / L, measure their arsenic content respectively, and calculate the recovery rate according to the original content, spiked amount and measured amount, the results are shown in Table 2. It can be seen from Table 2 that the standard addition recovery rate of this method is between 92.0% and 102.0%, indicating that this method has high accuracy.

[0038] The recovery rate of table 2 arsenic

[0039] sample

Numbering

content

(μg / L)

spiked

measur...

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Abstract

The invention discloses a method for testing arsenic content in tobaccos and tobacco products, which comprises following steps: adopting nitric acid-hydrogen peroxide to digest through microwaves, then taking chlorine lawrencium acid ammonium and citric acid as chemical promoting agents, using Zeeman to deduct backgrounds automatically, and adopting a graphite furnace atomic absorption method to test the arsenic content in tobacco samples. Results show that the detecting limit of the arsenic content is 0.28 um g / L, recovery ratio is between 92.0%-102.0%, and RSD is<3.0%. Agent species and quantity which are used for digesting are decreased greatly, analyzing time is shortened greatly, the invention is simple, rapid, and applicable to the rapid arsenic content tests of tobacco samples in batches, and the invention has good technique extension value.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical testing, and in particular relates to a method for determining the content of trace element arsenic in tobacco and tobacco products. Background technique [0002] Arsenic is a heavy metal that is harmful to the human body. Arsenic-containing substances are often used as insecticides, fungicides, herbicides, and defoliants in the soil, making the soil polluted by arsenic. High doses of arsenic will interfere with the energy metabolism of cells, thereby lead to the death of the organism. [0003] Trace elements and heavy metals in the soil will be absorbed by the tobacco plant during the growth of the tobacco plant, and can enter the human body through smoking, including arsenic. [0004] Therefore, it is necessary to quickly and accurately determine arsenic in cigarette products. Atomic fluorescence method and ICP-MS method are mostly used for the determination of arsenic in domesti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/44G01N1/28
Inventor 李峰沈光林胡静
Owner CHINA TOBACCO GUANGDONG IND
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