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American cockroaches effective fraction for anti-tumor prepared by macroporous adsorption resin and use

A technology of Periplaneta americana and effective parts, which is applied in the field of medicine and can solve the problems of expensive reversed-phase materials and other issues

Active Publication Date: 2008-07-09
KUNMING SINOWAY NATURAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, it is worth noting that the reusability of polyamide has a certain degree of limitation, and the price of reversed phase materials (such as RP-8 and RP-18) is relatively expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation of Extraction Parts from Periplaneta americana Alcoholic Extract Refined by Macroporous Adsorption Resin AB-8

[0026] 1.1 Preparation of the alcoholic extract of Periplaneta americana: Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of 55% ethanol, soak and extract at room temperature, extract 3 times, each time for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the alcoholic extract of Periplaneta americana.

[0027] 1.2 Preparation of different extracts of macroporous adsorption resin AB-8: 20 grams of alcoholic extract of Periplaneta americana, mixed with 30 grams of macroporous adsorption resin AB-8 to absorb and mix the sample, put it on the column, and first wash it with water until it is colorless. Continue to elute with 60% ethanol-water solution until it is colorless, take 10 ml of eluent, concentrate it to dryness under reduced pressure, and dry i...

Embodiment 2

[0028] Example 2: Preparation of Extraction Parts from Periplaneta americana Alcoholic Extract Refined by Macroporous Adsorption Resin D-101

[0029] 2.1 Preparation of the alcoholic extract of Periplaneta americana: same as Example 1.1.

[0030] 2.2 Preparation of different extracts from macroporous adsorption resin D-101: 20 grams of alcoholic extract of Periplaneta americana, mixed with 30 grams of macroporous adsorption resin D-101, loaded on the column, and eluted with water until colorless. Continue to elute with 60% ethanol-water solution until it is colorless, take 10 ml of the eluent, concentrate it to dryness under reduced pressure, and dry it in vacuum to obtain the extract (II) [Periplaneta americana alcohol extract D-101 column 60% alcohol wash liquid].

Embodiment 3

[0031] Example 3: Preparation of Extraction Parts from Periplaneta americana Alcoholic Extract Refined by Macroporous Adsorption Resin DA-201

[0032] 3.1 Preparation of the alcoholic extract of Periplaneta americana: same as Example 1.1.

[0033] 3.2 Preparation of different extracts from macroporous adsorption resin DA-201: 20 grams of alcoholic extract of Periplaneta americana, mixed with 30 grams of macroporous adsorption resin DA-201, loaded on the column, and eluted with water until colorless. Continue to elute with 60% ethanol-water solution until it is colorless, take 10 ml of the eluent, concentrate it to dryness under reduced pressure, and dry it in vacuum to obtain the extract (III) [Periplaneta americana alcohol extract DA-201 column washed with 60% alcohol liquid].

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PUM

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Abstract

The present invention relates to a periplaneta americana extract effective part with cytotoxic activity, a preparation method and a medical purpose thereof. The periplaneta americana extract effective part of the present invention is refined from fresh or dry blattidae periplaneta americana body which is extracted by alcohol water, processed for macroporous resin column chromatography and is eluted by alcohol solvent. The periplaneta americana extract effective part obtained by the present invention has obvious cytotoxicity and internal anticancer efficacy towards oral epidermoid carcinoma (KB) cell, poorly differentiated gastric abenocarcinoma tumour (BGC-823) cell, human chronic myelogonium leukaemia cancer (K562) cell and human myelogonium leukaemia cancer cell (HL-60) and can be used for preparing for a drug and a health care product for remedying the tumours.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular, the invention relates to an effective part of a Periplaneta americana extract with cytotoxic activity and antitumor function, a preparation method and a medical application thereof. Background technique [0002] Periplaneta americana (Periplaneta americana) belongs to the class Insecta, subclass Wings, order Blattata, family Blattadeidae, genus Periplaneta, commonly known as cockroach. It has survived on the earth for 400 million years, and is one of the most vigorous, oldest, and most successful insect groups in the world. According to reports, after the atomic bombing of Hiroshima, Japan, the first insect group to become active was the American cockroach. It can reproduce tenaciously for 400 million years, and the group is still very active in the face of thousands of various killing agents against it. It undoubtedly has special biologically active substances and its unique phy...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61K9/00A61P35/00
Inventor 刘光明何正春王晓雨赵昱巫秀美王熙才施贵荣肖怀蒋永新董光平
Owner KUNMING SINOWAY NATURAL PHARMA
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