Method for preparing tilapia fishskin polypeptide chelate zinc salt

A technology of tilapia and chelated zinc, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve problems such as difficult absorption and side effects, and achieve the effect of solving resource waste.

Inactive Publication Date: 2008-07-23
NANCHANG UNIV
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AI Technical Summary

Problems solved by technology

[0017] The purpose of the present invention is to provide a preparation method of fish skin polypeptide chelated zinc s

Method used

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Embodiment example

[0047] Weigh 100.0008g of washed tilapia skin, after degreasing treatment, soak it in 700ml of 0.1mol / L hydrochloric acid solution and 0.1mol / L sodium hydroxide solution for 1hr, wash with distilled water until neutral, and place it at 45℃ Under certain conditions, use 1000ml of distilled water to homogenize at a constant temperature for 12 hours, extract the supernatant by suction filtration, raise the temperature to 50°C-55°C, add 0.04671g of papain to hydrolyze for 2hr under the condition of adjusting the pH value to 6.0, and then heat it at 100°C Heated in a water bath for 20 minutes to terminate the enzymatic hydrolysis reaction to obtain a fish skin protein enzymatic hydrolysis solution, namely TSP-I solution. The degree of hydrolysis of tilapia fish skin protein was 12.3%.

[0048] The enzymolysis solution was debittered, decolorized and deodorized by using a compound decolorizing agent (3g / L activated carbon yeast mixed with cyclodextrin, etc.), and the supernatant was ...

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Abstract

The invention relates to a preparation method for polypeptide chelating zinc salt from the fish skin of tilapia, which is characterized in that the fish skin of tilapia is adopted as the material; the enzymatic method is adopted to prepare fish skin polypeptide (TSP-I); the water bath heating method is adopted to prepare the polypeptide chelating zinc salt from the fish skin of tilapia with the fish skin polypeptide liquid and zinc sulfate; and the chelating reaction conditions of the two are that: the content of polypeptide in the TSP-I solution is adopted as index; the mass ratio of polypeptide to zinc sulfate is 3 : 1; the chelating temperature is 60 DEG C; the pH value of reaction is 6.0; and the reaction time is 0.5 hr. The preparation method for polypeptide chelating zinc salt from the fish skin of tilapia has the advantages that the resource waste problem of tilapia leftover is solved; the high-efficiency preparation method of zinc supplement agent is provided; and the preparedpolypeptide chelating zinc salt from the fish skin is a popular and natural production with health function.

Description

technical field [0001] The invention relates to a preparation method of tilapia fish skin polypeptide chelated zinc salt. Background technique [0002] Zinc is an essential trace element for animals, and it is called a "life element" because of its extensive physiological and biochemical functions in the body. The biological functions of zinc in the human body are mainly manifested in the following aspects: [0003] (1) Participate in the composition of many metalloenzymes in the human body [0004] Zinc is a component of more than 200 kinds of enzymes in the body. There are zinc-containing enzymes in the six major enzymes (oxidoreductases, transferases, hydrolyzing intoxicants, lysing intoxicants, isomerases and synthetases); Many enzymes must be active and achieve maximum enzyme activity in the presence of zinc; at the same time, zinc can also maintain the structural configuration of the organic molecular ligands of some enzymes and act as a coenzyme in the enzyme reacti...

Claims

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Application Information

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IPC IPC(8): C07K1/18C12P21/06
Inventor 刘伟刘成梅梁汉萦梁瑞红涂宗财
Owner NANCHANG UNIV
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