Three-dimensional microcosmic appearance inclined scanning method and apparatus

Abstract

The invention relates to a three-dimensional micro-morphology oblique scanning method and a corresponding device. The method is characterized in that the position of a focus object is adjusted by three stepping motors controlled by a computer, ensuring the object always at the central place in the oblique scanning and obtaining image texture and high-level information of the focus surface of the object, then obtaining a lager three-dimensional morphology image through synthesis and restructure. The device comprises the computer, a microscope and a camera (1); wherein, the camera is arranged on the microscope, an object-carrying platform (13) of the microscope is provided with an object-carrying wedge-shaped platform (14), the object-carrying wedge-shaped platform and a drift angle of the object-carrying platform forms a oblique focus surface when the drift angle Beta is 5 to 40 degrees, the three-dimensional movement space coordinate of the object and the image of the camera at the space coordinate are inputted to the computer at the same time, then restructuring the three-dimensional micro-morphology high-level and texture information on the basis. The three-dimensional micro-morphology oblique scanning method and a corresponding device has the advantages of avoiding useless work in non-focus area, reducing scanning time and improving scanning speed and efficiency.

Description

technical field [0001] The invention relates to three-dimensional scanning, in particular to a method and device for oblique scanning of three-dimensional microscopic topography. Background technique [0002] At present, the principle of focal plane can be used to solve the three-dimensional image processing and reconstruction of microscopic cells, and the method of deconvolution and blind deconvolution is used to process the three-dimensional image of the fluorescence of biological cells by using a fluorescence microscope to improve the resolution of the image [2], Using this principle to design patents "ZL 2005200967777.9 (Notice No. CN2804794)" and "200510018921.1 (Publication No. CN1715987)", these can record three-dimensional surface information in the form of two-dimensional plane image sequences and one-dimensional height data. By analyzing these information Reconstruction can obtain intuitive three-dimensional surface information, and then use the three-dimensional s...

AI Technical Summary

Problems solved by technology

However, these methods scan an object longitudinally and vertically to obtain a three-dimensional image of only one area. During the scanning process, horizontal movement cannot be performed. If you want to obtain a larger three-dimensional image, you need to re-move a place and re-vertical Vertical scanning, and then splicing to get a larger three-dimensional image, most of the time in this process is to scan the unfocused surface, which is a waste of time and low efficiency

Images