Artificial breeding method of pimiento male sterile restoring line

A male sterility, restorer line technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of no successful transfer, no three-line seed production, weak growth, etc., and achieve stable yield and stability. The effect of improving sex, improving seed quality, and improving seed yield

Inactive Publication Date: 2008-07-30
HEFEI JIANGHUAI HORTICULTURE RES INST
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AI Technical Summary

Problems solved by technology

The hybridization rate of pepper varieties currently used in my country is less than 50%
[0006] The research on the male sterile three lines of pepper has been reported at home and abroad, and there is a precedent for the three-line matching of hot peppers in production, but this restorer line is found in nature, not many, and These pepper restorer lines are resources with weak growth and little commercial value, so the male sterile lines must be late-maturing pepper varieties with strong growth
However, there is no report on the three-line seed production of pepper × sweet pepper intermediate varieties widely used in pepper cultivation, that is to say, sweet pepper is not used as the male parent to realize three-line seed production
The main reason is that sweet pepper is used as a restorer line in the research of pepper fertility, and there is no precedent for successful transformation.

Method used

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  • Artificial breeding method of pimiento male sterile restoring line

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Embodiment

[0044]Through the Korean three-line seed variety CHAMPION, the gene expression is SMsms, and it is crossed with Shanghai Jimen sweet pepper 93-27-11. Using the restorer gene in the male parent of CHAMPION, according to theoretical calculations, it should have 25% of the restorer genes, 50 % fertile, select fertile offspring, eliminate sterile plants, that is, restore 50% of the gene. Always select fertile plants, which are the closest in traits to a single plant of Shanghai Jimen sweet pepper 93-27-11, and then continue to cultivate for 4 generations to ensure that 50% of the selected plants can always restore genes. This is the first step to ensure that the transgenic plants are more like 93-27-11 under the premise of ensuring 50% of the restored genes. The purpose of backcrossing is to improve the restoration gene so that it can finally achieve 100% fertility, that is, the genotype is SMsMs. Through 4 generations of backcrossing, combined with test crossing, the offspring of...

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Abstract

The invention relates to an artificial transferring breeding method for a bell pepper restoring line, which consists of the following two steps: hybridizing a Korean species CHAMPION with a Shanghai Qie Men pimento 93-27-11, and selecting a restorable offspring for the continuous backcross with the Shanghai Qie Men pimento 93-27-11 for four generations, while in the process of backcross, picking the plants with the agronomic characters most close to the Shanghai Qie Men pimento 93-27-11 and the spring being able to breed plants, and eliminating the infertile plants; then carrying out the individual plant selfing segregation, which is to pick 2-3 individual plants with higher offspring restoring rate to carry out the individual plant selfing segregation for four generations through hybridizing the individual plant with the infertile plant, thus leading the self-segregation individual plant with 100 percent offspring to be the fertile plant bell pepper restoring line 93-27-11C. The method of the invention can save the breeding cost by 58 percent and can increase the seed yield by 41.7 percent. As no male infertility is removed, the germen is not affected, which results in great improvement of seed output and stability. The purity of seed production is increased to 100 percent, while the income of every hectare is increased by 2700 Yuan.

Description

technical field [0001] The present invention relates to capsicum seed production. Background technique [0002] Capsicum is an important vegetable crop cultivated all over the world. At present, the sown area of ​​chili peppers in my country is about 1.3 million hectares per year, with an output of 27 million tons, accounting for about 46% of the world's total chili pepper production, and an output value of 27 billion yuan. The export of chili products has become one of the important growth points of foreign exchange earning from the export of economic crops in my country. The export volume of chili products accounts for about one-third of the world's total transaction volume, and the export volume ranks first in the world. [0003] Among all the cultivated pepper varieties in my country, the intermediate pepper cultivated by pepper × sweet pepper accounts for about 30% of the entire pepper cultivation area, that is, the cultivation area is about 400,000 hectares. [0004]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 戴祖云
Owner HEFEI JIANGHUAI HORTICULTURE RES INST
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