Artificial breeding method of pimiento male sterile restoring line
A male sterility, restorer line technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of no successful transfer, no three-line seed production, weak growth, etc., and achieve stable yield and stability. The effect of improving sex, improving seed quality, and improving seed yield
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[0044]Through the Korean three-line seed variety CHAMPION, the gene expression is SMsms, and it is crossed with Shanghai Jimen sweet pepper 93-27-11. Using the restorer gene in the male parent of CHAMPION, according to theoretical calculations, it should have 25% of the restorer genes, 50 % fertile, select fertile offspring, eliminate sterile plants, that is, restore 50% of the gene. Always select fertile plants, which are the closest in traits to a single plant of Shanghai Jimen sweet pepper 93-27-11, and then continue to cultivate for 4 generations to ensure that 50% of the selected plants can always restore genes. This is the first step to ensure that the transgenic plants are more like 93-27-11 under the premise of ensuring 50% of the restored genes. The purpose of backcrossing is to improve the restoration gene so that it can finally achieve 100% fertility, that is, the genotype is SMsMs. Through 4 generations of backcrossing, combined with test crossing, the offspring of...
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