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Method for detecting pork quality traits

A technology for pork and traits, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve problems such as unfavorable molecular mechanism, difficulty in screening candidate genes, and information loss

Inactive Publication Date: 2010-12-15
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

Therefore, analysis based only on coding genes will result in incomplete transcriptional profile information, which is very unfavorable for understanding the molecular mechanism of trait formation
It is very difficult to screen candidate genes based on this information

Method used

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  • Method for detecting pork quality traits

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Embodiment 1

[0020] Example 1. Establishment of a method for detecting pork quality traits by RFLP polymorphism and its effect verification

[0021] 1. Detection of single nucleotide polymorphisms in partial DNA sequences of pork quality-related genes

[0022] In the LongSAGE database (Tang Zhonglin, doctoral dissertation, Huazhong Agricultural University, 2006), a part of the differentially expressed tags without gene interpretation was screened out, and then the EST sequence was downloaded from the corresponding UniGene database, and performed using SeqMan software (Lasergene 6.0, USA) Contigs stitching. For these Contigs, use the ORFfinder software (NCBI) to predict the reading frame (Open read frame, ORF), keep the Contigs that have no coding potential or only contain <100aa, and perform Blastp comparison on these predicted small peptides, and further retain the contigs with the existing ones. There are contigs with no homology in various protein databases, and then use Blastn (NCBI) ...

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Abstract

The invention discloses a method of detecting the quality of pork. In the method, the gene type of the pig is determined by detecting that the ribonucleotide is C or G on the 451st site on the 51end of the sequence 1 or the 112th site on the 5 (1) end of the sequence 2; and the quality of pork is determined by the gene type; the method of determining the gene type of the pig is as follows: when the ribonucleotide is C on the 451st site on the 5 (1) end of the sequence 1, the homozygote gene type is AA; when the ribonucleotide is G on the 112th site on the 51 end of the sequence 2, the homozygote gene type is BB; the heterozygous gene type is AB; the method of determining the quality of pork via the gene type is as follows: the AA gene type pork tenderness and pH are higher than the AB gene type pork; the AB gene type AB gene type are higher than the BB gene type pork. The method of invention can be applied to detect the tenderness and pH value of the pig which indicate the quality of muscle, which provides a method of accurately and conveniently detecting the hereditary character for the molecular breeding of pig.

Description

technical field [0001] A method for detecting meat quality traits. Background technique [0002] Modern breeding technology has greatly improved the growth rate and lean meat rate of pigs. However, due to the antagonism between meat quality traits, growth traits and carcass traits in general, in those strains that have undergone high-intensity selection for growth rate and carcass lean meat percentage, it is often accompanied by a decline in pork quality. With the improvement of people's living standards, pork consumption no longer pursues lean meat alone, but high-quality pork with complete color, aroma and taste. Correspondingly, the breeding production of pigs is also developing towards the direction of breeding high-quality meat pigs. Developed countries have included meat quality traits as the breeding goals of pigs during 1996-2000. Therefore, breeding high-quality pigs with better muscle color, pH value, water retention, intramuscular fat and tenderness has always b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 李奎李勇唐中林杨述林
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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