A detection method of Radix Radix

A detection method and technology of Radix Radix are applied in the directions of measuring device, material separation, analysis of materials, etc., which can solve the problems of complex detection methods of Radix isatidis and difficult quality control, and achieve good accuracy and practical effect, high accuracy and simple method. Effect

Active Publication Date: 2015-12-09
LONGXI ZHONGTIAN PHARM CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention is that the detection method of Radix Radix is ​​complicated in the prior art, and the problem that the quality is difficult to control, and then provide a kind of method with high accuracy, simple and fast detection Radix Radix Radix

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  • A detection method of Radix Radix
  • A detection method of Radix Radix
  • A detection method of Radix Radix

Examples

Experimental program
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Effect test

Embodiment 1

[0039]Example 1 Determination of isatidisin, calycosin, formononetin, isatidis saponin Ⅱ and isatidis saponin Ⅲ content in Radix isatidis medicinal material

[0040] 1. Instruments and reagents

[0041] 1.1 Instrument

[0042] waters2695-2998 high performance liquid chromatography, ultrasonic cleaner (250W, 40kHz).

[0043] 1.2 Reagent

[0044] Guanosine reference substance (A0778, purity 98.4%, Tianjin Mark Biological Co., Ltd.), uridine reference substance (887-200001, China Institute for the Control of Pharmaceutical and Biological Products), adenosine reference substance (110879-200202, China Institute for Food and Drug Control ).

[0045] Methanol and acetonitrile are chromatographically pure, water is pure water, and ethanol is analytically pure.

[0046] 1.3 Medicinal material samples

[0047] Isatis root samples from various places were collected, and the batches of the medicinal materials tested were selected from: 2 batches in Shouyang, Longxi, marked as 1-2, Do...

Embodiment 2

[0080] Determination of total acid content of embodiment 2 Radix Radix Radix medicinal material

[0081] Isatis root samples from various places were collected, and the batches of the medicinal materials tested were selected from: 2 batches in Shouyang, Longxi, marked as 1-2, Dongjiabao, Shouyang, Longxi, Gansu, Caiziping, Shouyang, Longxi, Gansu, Huichuan, Weiyuan, Gansu , Gansu Lintao, Gansu Weiyuan 2 batches, marked as 1-2 respectively, Weiyuan Lianfeng Zhanpo Village 3 batches, respectively marked 1-3, Gansu commodities.

[0082] Accurately weigh 5 g of Radix isatidis, put it in a conical flask, add 70% ethanol, place it on a water bath, heat and reflux for extraction 3 times, each time 50 ml and reflux for 1.5 hours each time, filter, and concentrate the filtrate to a relative density of about 1.05 (60 ° C). Adjust the pH to 2-3 with hydrochloric acid, extract 3 times with ethyl acetate, recover ethyl acetate under reduced pressure to dryness, add 70% ethanol to dissolve ...

Embodiment 3

[0088] Example 3 Determination of Pesticide Residues in Radix Isatidis Medicinal Material

[0089] 1 Instruments and reagents

[0090] 1.1 Instrument

[0091] Clarus600 gas chromatography-mass spectrometry (US PE company), HS10260D ultrasonic extractor (Kunshan Ultrasonic Instrument Co., Ltd.), RE-52A rotary evaporator (Shanghai Yarong Biochemical Instrument Factory).

[0092] 1.2 Reagent

[0093] Dichlorvos, methamidophos, acephate, tetrachloronitrobenzene, hexachlorobenzene, α-666, β-666, γ-666, δ-666, omethoate, dimethoate Azinon, pentachloronitrobenzene, monocrotophos, difenthion, phosphamide I, dimethoate, heptachlor, pentachloroaniline, chlorothalonil, chlorpyrifos-methyl, aldrin, captan, phosphamide II. Methyl parathion, pirimiphos-methyl, methyl pentachlorophenyl sulfide, metalaxyl, triadimefon, chlorpyrifos, malathion, fenitrothion, parathion, pendimethalin , cis epoxy heptachlor, trans epoxy heptachlor, triadimenol A, triadimenol B, trans chlordane, cis endosulfa...

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Abstract

The invention belongs to the field of quality inspection of traditional Chinese medicine, and particularly relates to a quality detection method of isatis root. The method comprises the steps of determining contents of uridine, guanosine, adenosine and total acid contained in the isatis root and determining residual quantity of pesticide on the isatis root. By adopting the detection method, the technical problems of the traditional physical and chemical property identification method that the operation is complicated and limited and the quality is difficult to control can be solved, a detection method with high accuracy, simple and rapid operation and low cost is provided, and the application prospect and economic benefit are good.

Description

technical field [0001] The invention belongs to the field of quality detection of traditional Chinese medicinal materials, and in particular relates to a quality detection method of Radix isatidis medicinal material. Background technique [0002] Banlangen, commonly known as indigo root, blue indigo root or Daqing root, is a traditional Chinese medicine. Produced all over China. Isatidis root is divided into northern isatid root and southern isatid root. The source of northern isatid root is the root of cruciferous plants Isatis indigo and Daqing grass; southern isatid root is the rhizome and root of acanthaceae plant Malan. It has the effects of clearing away heat and detoxification, cooling blood and reducing swelling, and relieving sore throat. Banlangen is a medicinal material that has been used in a large amount and has been sold for a long time. Throughout the history of Banlangen, we can see that its sales are growing rapidly. Radix isatidis contains a variety of c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 宋平顺李登鹏陈杰韩斌李波赵建邦李士博李冬华张明童
Owner LONGXI ZHONGTIAN PHARM CO LTD
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