Core-peptide composition with physiological activity and its preparation method and application
A technology of peptide complex and physiological activity, which is applied in the field of biomedicine to achieve the effect of increasing the level of free testosterone, significant purification effect, and easy operation
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Embodiment 1
[0048] Take 100g of red snail tissue (trunk, liver, reproductive organs) and mash it in a frozen state, add 10 times the amount of 95% ethanol to extract for 1 hour, filter, then stir and extract with 50% acidic ethanol for 3 hours, filter, combine the two extracts, Let cool, precipitate out, pour out the supernatant, concentrate, treat with 3 times the amount of hexane, discard the hexane layer, purify the lower lipoprotein layer with 2% activated carbon, ultrafilter, concentrate, and keep the operating temperature below 40 ℃, add vitamin E to make the final concentration to 0.03%, concentrate under reduced pressure to remove the solvent, obtain the crude product, and then use sephadex LH20 column chromatography to separate, the volume ratio is 5: 3 methanol-water composite solvent (use acetic acid to adjust the eluent to be acidic) ) to elute the nucleopeptide complex.
[0049] After chemical analysis and detection, the obtained nucleopeptide complex components include per m...
Embodiment 2
[0051] Take 100g of the whole tissue of the red snail and mash it in the frozen state, add 10 times the amount of 50% ethanol, adjust the pH value to not less than 4.5 with acetic acid, extract it in a container with mixing and stirring function for 2 hours, filter, concentrate, and let it cool The precipitate was precipitated, the supernatant was poured out, treated with 3 times the amount of hexane, the hexane layer was discarded, the lower lipoprotein layer was purified with 2% activated carbon, ultrafiltered, concentrated, the operating temperature was always kept below 40°C, and 0.02% Vitamin E was concentrated under reduced pressure to remove the solvent to obtain a crude product, which was then separated by sephadex LH20 column chromatography and eluted with a methanol-water composite solvent with a volume ratio of 5:2 to obtain a nucleopeptide complex.
[0052] After chemical analysis and detection, the obtained nucleopeptide complex components include per milliliter: 8...
Embodiment 3
[0054] Take 100g of snail tissues (trunk, liver, reproductive organs) and crush them in a frozen state, add 8 times the amount of 80% ethanol, adjust the pH value to not less than 4.5 with acetic acid, stir and extract for 3 hours, filter, and concentrate to 1 / 2 of the original volume 10. Let the precipitate cool down, pour out the supernatant, treat with 3 times the amount of hexane, discard the hexane layer, purify the lower lipoprotein layer with 2% activated carbon, ultrafilter, concentrate, and keep the operating temperature below 40°C. , add 0.03% vitamin E, concentrate under reduced pressure to remove the solvent to obtain the crude product, then use sephadex LH20 column chromatography to separate, the volume ratio is 5:4 ethanol-water composite solvent (use acetic acid to adjust the eluent to be acidic) and elute to obtain the nucleus peptide complex.
[0055] After chemical analysis and detection, the obtained nucleopeptide complex components include per milliliter: 8...
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