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Ocean cephalopod skin glue collagen and preparation method thereof

A technology of collagen and cephalopods, which is applied in the field of marine cephalopod type I collagen products and its preparation, can solve the problems of unseen marine cephalopod original protein patent reports, etc., to avoid new pollution, high Safety and value-added effects

Inactive Publication Date: 2012-06-13
SHANGHAI FISHERIES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Unfortunately, there is currently no patent report on marine cephalopod proprotein

Method used

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  • Ocean cephalopod skin glue collagen and preparation method thereof

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Effect test

Embodiment 1

[0033] 1. Raw material pretreatment

[0034] Fresh squid skin, washed with distilled water;

[0035] 2. Use eluate I to remove water-soluble impurities

[0036] Pound the cleaned squid skin with a tissue masher, add 10 times the amount of eluate I to homogenize, centrifuge (5000×g, 15min) to get the precipitate, then add eluate I to repeat the treatment twice;

[0037] 3. Use eluent II to remove salt-soluble impurities

[0038] Add 10 times the amount of eluate II to the precipitate obtained through the above steps, homogenize, stir and extract for 24 hours, centrifuge to collect the precipitate, and wash the precipitate twice with clean water;

[0039] 3. Use the extract to extract collagen

[0040] Add 10 times the amount of extract to the above precipitation, stir and extract for 48 hours, centrifuge to take the supernatant to obtain the crude collagen extract;

[0041] 4. Salting out of collagen

[0042] Add solid NaCl to the crude collagen extract to make the final ...

Embodiment 2

[0048] Wash the fresh or thawed cuttlefish skin with water, cut into pieces, homogenize, add 5 times the amount of eluate I for post-treatment, centrifuge to remove the supernatant, add 10 times the amount of eluate II to the precipitate, Stir and extract for 16h. Take the precipitate by centrifugation, wash the precipitate with clean water, and then use 15 times the amount of the extract, stir and extract for 56 hours to obtain a crude collagen extract. Add solid NaCl to make the final concentration reach 2.5M, centrifuge to discard the supernatant, dissolve the precipitate with 0.5M acetic acid, and perform dialysis, use 0.5M acetic acid as the dialyzed external fluid until the dialysate is free of Cl - So far, after being freeze-dried, squid skin collagen product is obtained, its molecular weight is 330KD, intrinsic viscosity is 18dL / g, denaturation temperature is 30°C, and every 1000 amino acid residues contains glycine, proline and Hydroxyproline is 330, 90, 70 respectiv...

Embodiment 3

[0050] Thaw the frozen squid skin in the storage room of the refrigerator, clean it with deionized water, then add 10 times the amount of eluate I, and homogenize it in a tissue masher. Centrifuge (2000×g, 15min), remove the supernatant, and stir and leach the precipitate with 15 times the amount of eluate II for 48h. Take the precipitate by centrifugation, wash the precipitate with deionized water for 3 times, add 20 times the amount of extract containing 1% pepsin, stir and extract for 72 hours, and centrifuge to get the supernatant, which is the crude collagen extract. Add solid KCl to make the final concentration up to 2.5M, centrifuge to remove the supernatant, dissolve the precipitate with 0.5M acetic acid, and perform dialysis treatment. The external fluid of the dialysis is 0.5M acetic acid. A squid skin collagen product is obtained after drying. After SDS-PAGE and amino acid analysis, it was determined to be type I collagen, and the molecular type was: [α1(I)] 2 The...

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Abstract

The invention pertains to biological resources and environmental technology, in particular to a marine caphalopoda animal I collagen protein product and the preparation method thereof. The invention discloses the marine caphalopoda animal collagen protein, the collagen protein is extracted from the skin of marine caphalopoda animals, the collagen protein is white or white with the light brown and is the I type collagen protein, the molecular type is (Alpha 1)2Alpha2 type, the molecular weight of the main peptide chain is 300 to 330KD, the intrinsic viscosity is 12 to 18dL / g, the denatured temperature is 15 to 30 DEG C, and every 1000 amino acid residues contain 280 to 330 parts of glycine, 80 to 90 parts of proline and 60 to 70 parts of hydroxyproline respectively. The preparation method takes squid skin which is one of the processing waste of the seafood as raw material for preparing the I type collagen protein; an edible biochemical reagent, mild preparation conditions and simple physical method are adopted for carrying out the extraction of the collagen protein in the squid skin; the obtained collagen protein product dose not have the residue problem of the toxic and harmful reagents, which has very high safety; therefore, in addition to the general usages of the collagen protein, the marine caphalopoda animal I collagen protein product can also be directly applied in the industries of general foods, special foods, functional foods and biomedicine; the usage of the method for preparing the collagen protein is environmental-friendly, which does not discharge toxic and harmful substances to the environment and can avoid the new pollution to the environment of certain preparation methods.

Description

technical field [0001] The invention belongs to the technical field of biological resources and environment, and in particular relates to a marine cephalopod type I collagen product and a preparation method thereof. Background technique [0002] Collagen is an important structural protein, which widely exists in supporting tissues such as bones, epidermis and muscle tendons of multicellular animals. Collagen is rich in glycine, proline and hydroxyproline, and there are physiologically active peptides in its hydrolyzate. Due to its weak antigenicity and good tissue compatibility, collagen and its hydrolyzed products have good application prospects in medical treatment, health care and beauty, from edible and photographic gelatin, to sausage packaging and functional food additives , have a wide range of uses. The specially treated soluble collagen is also used in medical and health fields such as burns, trauma, cosmetology, orthopedics, tissue repair, and wound hemostasis. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C07K1/14
Inventor 刘承初秦玉青王慥
Owner SHANGHAI FISHERIES UNIV
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