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Preadipocyte heterologous vaccine and preparation method and application thereof

A preadipocyte and xenogeneic technology, applied in the field of preadipocyte xenogeneic vaccine and its preparation, can solve the problems of unacceptable, high risk, and no feasibility of clinical implementation, etc.

Inactive Publication Date: 2010-04-14
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] It can be seen that the neuropeptide Y and leptin that can induce adipocyte apoptosis found in the current research require surgical intraventricular (i.c.v.) injection to have the therapeutic effect of inducing adipocyte apoptosis. This kind of complicated brain surgery Not only expensive, but also risky, generally unacceptable to patients, and basically not feasible for clinical implementation

Method used

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  • Preadipocyte heterologous vaccine and preparation method and application thereof
  • Preadipocyte heterologous vaccine and preparation method and application thereof
  • Preadipocyte heterologous vaccine and preparation method and application thereof

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Embodiment Construction

[0012] Technical scheme of the present invention is as follows:

[0013] The preadipocyte heterogeneous vaccine of the present invention is a preadipocyte heterogeneous vaccine produced by using mouse 3T3-L1 preadipocytes as the preadipocyte source. It can be used as a cross-antigen for heterogeneous immunity, stimulate the body's immune system, and generate an immune response to the body's preadipocytes.

[0014] Preadipocyte heterogeneous vaccine preparation method of the present invention, the steps are as follows:

[0015] Mouse 3T3-L1 preadipocytes were cultured on DMEM medium containing 10% fetal bovine serum, 100 U / mL penicillin, and 0.1 mg / mL streptomycin. When the mouse 3T3-L1 preadipocytes reached 70 %, digested with trypsin and passaged. The preadipocytes cultured in vitro were collected and washed 3 times with PBS. Prepare 3% paraformaldehyde with PBS, pH 7.4, as a fixative solution, and fix the cells at 4°C for 24h. Wash 3 times with PBS and incubate at 37°C f...

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Abstract

The present invention relates to a preadipocyte heterogenous bacterin and a preparation method and purposes thereof, which belongs to the biological technical field. The preadipocyte system 3T3-L1 ofamouse is adopted as the source of a bacterin cell of the preadipocyte heterogenous bacterin of the present invention; the preparation of the bacterin is that cells are increased by culture in vitro,p araformaldehyde is used for fixation; the residual paraformaldehyde is washed and discarded, and cell suspension is prepared to obtain the preadipocyte heterogenous bacterin. The preadipocyte heterogenous bacterin of the present invention can be immunized actively, prevent the occurrence of the adiposity and is used for remedying the adiposity.

Description

technical field [0001] The invention relates to a preadipocyte heterogeneous vaccine and its preparation method and application, belonging to the field of biotechnology. Background technique [0002] In recent years, with the development of my country's social economy, the improvement of people's material living standards and the change of lifestyle, the incidence of obesity has been increasing year by year. Obesity is a global chronic disease that seriously threatens human health. It is now known that obesity is an important risk factor for many chronic diseases and psychosocial disorders such as hypertension, hyperlipidemia, diabetes, cardiovascular disease, and sleep apnea syndrome. Important global health problem with national financial burden. [0003] According to the regulations of the World Health Organization, when the intake of calories exceeds the body's consumption and is stored in the body in the form of fat, resulting in body mass index (BMI): weight (Kg) / hei...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61P3/04
Inventor 来庆国高振南徐欣袁奎封
Owner SHANDONG UNIV
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