Panax japonicus polysaccharides, preparation method and use thereof

A technology of bamboo ginseng polysaccharides and heteropolysaccharides, which can be used in food preparation, application, allergic diseases, etc., can solve the problems of curative effect restrictions and changes, and achieve the effects of low cost, improving immune function, and simple and easy methods

Inactive Publication Date: 2010-07-28
WUHAN UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, if bamboo ginseng is directly used as traditional Chinese medicine, the curative effect is restricted, because bamboo ginseng contains many components, and the effects of various components are also different, among which polysaccharides include β-glucan, β-mannan, α- Mannan as well as heteropolysaccharides, and varies with extraction conditions

Method used

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  • Panax japonicus polysaccharides, preparation method and use thereof
  • Panax japonicus polysaccharides, preparation method and use thereof
  • Panax japonicus polysaccharides, preparation method and use thereof

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Embodiment Construction

[0015] The technical solution of the present invention will be further described below in conjunction with specific examples.

[0016] Reflux the dried and crushed Panax ginseng produced in Enshi, Hubei with ethyl acetate and acetone for 4-8 hours respectively to remove fat and some small molecular impurities. Soak 1-10L of aqueous solution at 10-30°C and extract with stirring for 5-24 hours, and repeat the extraction for 2-5 times. The mixed slurry was separated and precipitated by high-speed centrifugation, and the upper layer solutions were combined and then concentrated by a rotary evaporator. During concentration, a small amount of n-octanol was added to remove air bubbles. The concentrated solution was deproteinized by the Sevag method, after deproteinization, ammonia water was added to adjust the pH of the solution to 7-10, and then H 2 o 2 1 to 4 days for decolorization. Then dialyze with tap water and distilled water for 2-5 days in turn, freeze-dry and then reflux...

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Abstract

The invention discloses panax japonicus polysaccharide. The main chain structure thereof is Beta-1 to 4-D glucan and heterosaccharide containing mannan, galactan and xylan branched chain, the weight-average molecular weight of the panax japonicus polysaccharide is 5,000 to 200,000, the intrinsic viscosity at 25 DEG C is 1 to 50ml / g, and the mole ratio of the three elements of C: H: O is 1: 2: 1. The method for preparing the panax japonicus polysaccharide comprises the following steps: after being dried, smashed and skimmed, the panax japonicus is extracted by neutral salt water solution or pure water, and the white panax japonicus polysaccharide which can be easily dissolved in water, be dissolved in DMSO and not be dissolved in methanol, ethanol and acetone is obtained by separation, saponin removal, decolorization, dehydration, protein removal, desalination and drying. The panax japonicus polysaccharide which is prepared by the invention has higher anti-tumor activity and promotion of immune activity, which can be used for preparing drugs or health care products for improving immune function and anti-tumor. The method for preparing the product is simple and easy to operation, and the sources of the needed raw materials are wide.

Description

technical field [0001] The invention relates to a bamboo ginseng polysaccharide and its preparation method and application, which belong to the fields of polymer chemistry and physics, and also belong to the fields of biology, pharmacy, food and health products. Background technique [0002] Rhizoma Panacis Japonici, or bamboo ginseng, white notoginseng, pearl ginseng, etc., is the bamboo whip-shaped rhizome of Araliaceae plant Panax japonicus C.A.Mey, mainly produced in my country. In the south, it is a traditional Chinese medicine in our country. At present, the research on the chemical constituents contained in the rhizome of Panax ginseng is limited to chikusetsu-saponin, ginsenoside, and notoginseng saponin in the literature at home and abroad. 2 (notoginsenoside R 2 ) and pseudoginsenoside F 11 (pseudo-ginsenoside F 11 ), and the polysaccharides of bamboo ginseng have not been studied. Bamboo ginseng is a special resource in our country. It is used as medicine, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P35/00A61P37/04A23L1/30A23L33/105
Inventor 黄志萍张俐娜
Owner WUHAN UNIV
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