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Use of Erythrina bark extract in preparing antineoplastic medicine

A technology of anti-tumor drugs and extracts, applied in the field of application of pituitary bark extracts in the preparation of anti-tumor drugs, can solve problems such as applications that have not been reported, and achieve the effect of low cost and simple method

Inactive Publication Date: 2008-11-19
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the application of Pittogonia bark in the preparation of antitumor drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the preparation of Pittogonia bark extract

[0020] Get Pittogonia bark 200g (Beijing Tongrentang Co., Ltd.), mix it with 95% ethanol (ethanol: water=95: 5, volume ratio) with the weight and number ratio of 1: 3 (Pittogonia bark: 95% ethanol) , reflux extraction at 60°C for 3h, suction filtration, and collect the first reflux extract 1 and filter residue 1; mix all the filter residue 1 obtained with 95% ethanol, the amount of 95% ethanol is the same as the first time, at 60 Under the condition of reflux for 3 hours, collect the second reflux extract 2 and filter residue 2, and combine the extract 2 with the extract 1; mix all the obtained filter residue 2 with 95% ethanol, and the amount of 95% ethanol is the same as that of the first The same as the second time, reflux extraction at 60°C for 3h, suction filtration, and collect the third reflux extract 3; extract 1, extract 2 and extract 3 were combined, and the obtained extract was concentrated under redu...

Embodiment 2

[0021] Embodiment 2, the application of Pittogonia bark extract in inhibiting the growth of tumor cells

[0022] The logarithmically grown tumor cells (human liver cancer cell HepG-2, human liver cancer cell BEL-7402, human colon cancer cell HCT-8, human lung cancer cell A-549) were digested with 0.25% trypsin, and then treated with 10% (volume percentage content) DMEM complete medium of fetal bovine serum suspends cells, makes cell suspension, and dilutes each tumor cell to 1.0×10 respectively 5 cells / mL, and then inoculated into 96-well culture plate, 200uL per well, each tumor cell was inoculated into 3 wells respectively, at 37°C, 5% CO 2 Cultivate in an incubator for 24 hours; then pour out the culture solution in each well.

[0023] The Pittogonia extract obtained in Example 1 was dissolved in DMSO, and then diluted with DMEM complete medium to obtain the Pittogonia extract solution, so that the final concentration of the Pittogonia extract was 50ug / mL, and the DMSO Th...

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PUM

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Abstract

The invention discloses a new use of erythrina bark extract and particularly relates to the application of erythrina bark extract in preparation of antineoplastic drugs. The extract is prepared according to the following method: the erythrina bark extract is extracted from erythrina bark with water and / or an organic solvent as an extractant. The erythrina bark extract provided by the invention is very important for the preparation of antineoplastic medicaments, in particular anti-liver cancer medicaments, anti-lung cancer medicaments, and anti-colon cancer medicaments.

Description

technical field [0001] The invention relates to the application of Pittogonia bark extract in the preparation of antitumor drugs. Background technique [0002] Liver cancer is one of the common malignant tumors in our country, and it is the second cancer "killer" in our country, which is common in middle-aged men. Because of its high degree of malignancy and rapid disease progression, patients generally have no discomfort in the early stage, but once symptoms appear and go to the doctor, it is already in the middle and late stages. Therefore, the treatment of liver cancer is very difficult, and the average patient's survival time after onset is only 6 months, which is called "the king of cancer". According to the survey results, the top three new cancers in men are lung cancer, colon cancer, and nasopharyngeal cancer, and breast cancer, colon cancer, and lung cancer in women, which fully demonstrates the harm of lung cancer and colon cancer in the new cancer group sex. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P35/00A61K125/00A61K129/00
Inventor 唐亚林谭莉戴玮刘岩向俊锋
Owner INST OF CHEM CHINESE ACAD OF SCI
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